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Ectoderm mesenchymal stem cells promote osteogenic differentiation of MC3T3-E1 cells by targeting sonic hedgehog signaling pathway

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Abstract

Background

Despite their high repair capability, bone defects still present a major challenge in orthopedic tissue engineering. Osteoblast differentiation is central to the treatment of bone defects.

Methods and results

We used nasal mucosal-derived ectoderm mesenchymal stem cells (EMSCs) to promote osteogenic differentiation by co-culturing MC3T3-E1 cells. Our results showed that MC3T3-E1/EMSCs co-culture upregulated bone-related proteins and transglutaminase 2 (TG2) and increased alkaline phosphatase (ALP) activity and bone nodule formation relative to controls. Furthermore, our results showed that EMSC-derived sonic hedgehog (Shh) accounted for the enhanced MC3T3-E1 differentiation because inhibiting Shh signaling substantially reduced osteogenic differentiation.

Conclusion

Altogether, these results suggest that EMSCs differentiated into osteoblast cells and supported MC3T3-E1 differentiation. Thus, EMSCs may be a promising cell source for treating bone-related diseases.

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Data availability

For availability of data and materials, please contact the Xijiang Zhao for all data requests.

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Acknowledgements

We thank the University Ethics Committee for their kind guidance in the animal experiments.

Funding

This study was supported by the National Natural Science Foundation of China (Grant No. 81571830). This study was also supported by the Special fund project for the development of health science and technology in Nanjing (Grant No. YKK19129) and (Grants No. JYL20180040).

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Author notes

  1. Lu Bian, YiQing Wu and Jiawei Wu have contributed equally to this work.

Authors and Affiliations

  1. Wuxi School of Medicine, Jiangnan University, Wuxi, Jiangsu Province, China

    Lu Bian, YiQing Wu, Jiawei Wu, Peng Zhao & Xijiang Zhao

  2. Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu Province, China

    Lu Bian, YiQing Wu, Jiawei Wu, Peng Zhao & Xijiang Zhao

Authors
  1. Lu Bian
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  2. YiQing Wu
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Contributions

Study concept: LB and YW; data curation: JW; formal analysis: LB and YW; funding acquisition: PZ and XZ; methodology: JW; resources: YW, JW, and LB; writing and original draft: PZ and XZ; writing, review and editing: LB and XZ. All authors approved the final version of the paper.

Corresponding authors

Correspondence to Peng Zhao or Xijiang Zhao.

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The authors declare that they have no conflict of interest.

Ethical approval

All of the animal procedures were approved by the Jiangnan University Animal Care and Ethics Committee, and the International Guidelines for Animal Research were strictly followed in this study.

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Bian, L., Wu, Y., Wu, J. et al. Ectoderm mesenchymal stem cells promote osteogenic differentiation of MC3T3-E1 cells by targeting sonic hedgehog signaling pathway. Mol Biol Rep 50, 1293–1302 (2023). https://doi.org/10.1007/s11033-022-08022-8

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  • DOI: https://doi.org/10.1007/s11033-022-08022-8

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