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Inheritance of androgenesis response in pepper

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Abstract

Background

Anther culture has become an important part of pepper breeding. Response to haploidy via androgenesis is highly genotype-specific. However, studies on the inheritance of response to anther culture are lacking. Therefore, the study aimed to determine the inheritance of androgenesis.

Methods and results

The plant material included crosses involving Capsicum annuum L. (253 A, and Inan3363) X C. chinense PI 159,236 populations. To estimate the heritability of the trait, two pepper lines with very high androgenesis responses were crossed with PI 159,236, a non-responsive accession. The androgenesis response was phenotyped using the parents, F1, F2, BC1P1, and BC1P2/P3 created through reciprocal crosses. Using variance components, the number of genes controlling the trait, broad (H2 = 0.97), and narrow sense heritabilities (h2 = 0.20), genetic variance (VG=113.8), the additive (VA=23.9), and the dominance gene variances (VD = 89.9), as well as the environmental variance (VE = 3.6) were calculated. Additive and dominant gene effects were 21% and 79%, respectively. Results derived from two different populations showed that the number of genes controlling the trait was between 1.96 and 2.46, and H2 = 0.96–0.97, h2 = 0.20–0.65, VG=91.8-113.8, VA, = 23.9–62.7, VD = 29.1–89.9, and VE=3.5–3.6 were calculated. The X2 analysis indicated that the most suitable one is the 9: 3: 4 epistatic genetic model (X2 = 2.13, P = 0.343, N = 155).

Conclusions

Results obtained from two different populations indicate the existence of a few major genes for response to androgenesis in pepper. Elucidating the inheritance of androgenesis is expected to pave the way for tagging the gene(s) in the pepper genome.

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Data availability

The datasets used for the current study are available from the corresponding author on reasonable request.

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Funding

This study was supported by the Republic of Turkey Ministry of Agriculture and Forestry General Directorate of Agricultural Research and Policies (TAGEM/BBAD/12/A09/P01/03) and Cukurova University Scientific Research Projects Coordinating Office (FDK-2016-2414) financially.

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Authors and Affiliations

  1. Alata Horticultural Research Institute, 33740, Mersin, Turkey

    Nihal Denli, Atilla Ata & Davut Keleş

  2. Department of Agricultural Biotechnology, Faculty of Agriculture, Akdeniz University, 07070, Antalya, Turkey

    Nedim Mutlu

  3. Department of Horticulture, Faculty of Agriculture, Cukurova University, 01330, Adana, Turkey

    Hatıra Taşkın

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Contributions

Nihal Denli was responsible for writing the manuscript and for conducting and collecting data and interpretation of data. Atilla Ata was responsible for conducting and collecting data. Davut Keleş was responsible for the interpretation of data. Nedim Mutlu was responsible for the interpretation of data and for writing the manuscript. Hatıra Taşkın was responsible for the interpretation of data and for writing the manuscript. All authors read and approved the final manuscript.

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Correspondence to Nihal Denli.

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Denli, N., Ata, A., Keleş, D. et al. Inheritance of androgenesis response in pepper. Mol Biol Rep 49, 11601–11609 (2022). https://doi.org/10.1007/s11033-022-07876-2

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