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Trehalose biosynthetic pathway regulates filamentation response in Saccharomyces cerevisiae

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Abstract

Background

Diploid cells of Saccharomyces cerevisiae undergo either pseudohyphal differentiation or sporulation in response to depletion of carbon and nitrogen sources. Distinct signaling pathways regulate filamentation and sporulation in response to nutrient limitation. How these pathways are coordinated for implementing distinct cell fate decisions in response to similar nutritional cues is an enigma. Although the role of trehalose pathway in sporulation has been extensively studied, it’s possible role in pseudohyphal differentiation has been unexplored.

Methods and results

Briefly, tps1 and tps2 mutants were tested for their ability to form pseudohyphae independently as well as in the background of GPR1 and RAS2 mutations. Here, we demonstrate that disruption of TPS1 but not TPS2 inhibits pseudohyphae formation. Interestingly, deletion of GPR1 suppresses the above defect. Further genetic analysis revealed that TPS1 and TPS2 exert opposing effects in triggering filamentation.

Conclusion

We provide new insights into the role of an otherwise well-known pathway of trehalose biosynthesis in pseudohyphal differentiation. Based on additional data we propose that downstream signaling, mediated by cAMP may be modulated by nutrient mediated differential regulation of RAS2 by TPS1 and TPS2.

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Acknowledgements

This work was supported by funding awarded to Dr. Revathi Iyer, by the Department of Science and Technology, India, under the DST WOS-A scheme (SR/WOS-A/LS-58/2017). We thank Prof. R. Patkar, for graciously permitting the use of his laboratory facilities to study the effect of cAMP on the the ras2 mutant.

Funding

This work was supported by funding awarded to Dr. Revathi Iyer, by the Department of Science and Technology, India, under the DST WOS-A scheme (Grant No. SR/WOS-A/LS-58/2017).

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  1. Laboratory of Molecular Genetics, Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai, Maharashtra, 400 076, India

    Revathi Iyer & Paike Jayadeva Bhat

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  1. Revathi Iyer
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Both authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by Dr. RI. The first draft of the manuscript was written by Dr. RI. Both authors read and approved the final manuscript.

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Correspondence to Revathi Iyer.

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Iyer, R., Bhat, P.J. Trehalose biosynthetic pathway regulates filamentation response in Saccharomyces cerevisiae. Mol Biol Rep 49, 9387–9396 (2022). https://doi.org/10.1007/s11033-022-07792-5

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