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Genomic analysis of arginine vasopressin gene in riverine buffalo reveals its potential association with silent estrus behavior

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Abstract

Background

Poor estrus expression behavior causes suboptimal reproductive efficiency through poor conception rate. Various signaling pathways are involved in estrus expression but arginine vasopressin (AVP) gene with oxytocin predominantly regulates estrus behavior. This study aimed to perform genomic characterization and evolutionary dynamics of AVP gene through association testing of the novel polymorphic loci and comparative genomic analysis to explore the potential effect of AVP gene on estrus behavior of Nili-Ravi buffaloes.

Methods and results

198 Nili-Ravi buffaloes were screened for the quest of novel polymorphism in the AVP gene. In exon-1, five polymorphic sites were detected including deletion of two (c.47delA and c.57delA) nucleotides that caused drastic variation in subsequent amino acid sequence due to frame shift including functional short peptide of nine residues. The 3-D structure revealed a loss of transmembrane loop between 16 and 31 residues in Nili-Ravi buffalo AVP protein sequence, suggesting that missing loop apparently reduced the gene functionality in Nili-Ravi buffalo by inhibiting cellular reactions and muting the animal estrus cyclicity. Three polymorphisms detected in AVP gene were significantly associated with silent estrus (P < 0.05). The comparative genomic analysis revealed that AVP gene is present on chromosome 14 having one conserved motif (Neurohypophysial) in buffalo.

Conclusions

This study suggested the potential use of polymorphic sites as promising genetic markers for selection of buffaloes with pronounced estrus expression.

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Author information

Author notes

  1. Maryam Javed, Asif Nadeem and Faiz-ul Hassan have contributed equally to this work.

Authors and Affiliations

  1. Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore, Pakistan

    Maryam Javed & Huma Mujahid

  2. Department of Biotechnology, Virtual University of Pakistan, Lahore, Pakistan

    Asif Nadeem

  3. Institute of Animal and Dairy Sciences, Faculty of Animal Husbandry, University of Agriculture, 38040, Faisalabad, Pakistan

    Faiz-ul Hassan

  4. State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, 530005, Nanning, China

    Saif ur Rehman

Authors
  1. Maryam Javed
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  2. Asif Nadeem
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  3. Faiz-ul Hassan
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  4. Huma Mujahid
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  5. Saif ur Rehman
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Contributions

“Conceptualization, MJ, and FH, and AN; resources, MJ; data curation, FH and AN, and MJ; methodology, MJ, and DA; project administration, MJ and FH; Experiment, AN and MJ and DA; software, FH, DA.; supervision, MJ; writing—original draft preparation, FH AN and MJ; writing—review and editing, FH, AN, MJ; “All authors have read and agreed to the published version of the manuscript.”

Corresponding author

Correspondence to Maryam Javed.

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Conflict of interest

“The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest”.

Ethical approval

Phlebotomy was performed by considering institutional ethical guidelines. The study was approved by the Institutional Review Committee (RC/710).

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The study does not involve human tissue samples.

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Javed, M., Nadeem, A., Hassan, Fu. et al. Genomic analysis of arginine vasopressin gene in riverine buffalo reveals its potential association with silent estrus behavior. Mol Biol Rep 49, 9315–9324 (2022). https://doi.org/10.1007/s11033-022-07776-5

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  • DOI: https://doi.org/10.1007/s11033-022-07776-5

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