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The correlation of long non-coding RNAs IFNG-AS1 and ZEB2-AS1 with IFN-γ and ZEB-2 expression in PBMCs and clinical features of patients with coronary artery disease

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Abstract

Background

Aberrant expression of long non-coding RNAs (lncRNAs) can contribute to the pathogenesis of coronary artery disease (CAD). In this study, we aimed to evaluate the expression of lncRNA interferon γ-antisense 1 (IFNG-AS1), zinc finger E-box binding homeobox 2 antisense RNA 1 (ZEB2-AS1), and their direct target genes (IFN-γ and ZEB2, respectively) in peripheral blood mononuclear cell (PBMC) from CAD and healthy individuals.

Methods and results

We recruited 40 CAD patients and 40 healthy individuals. After doing some bioinformatics analyses, the expressions of IFNG-AS1/ ZEB2-AS1 lncRNAs and IFN-γ/ ZEB2 in PBMCs were measured using quantitative real-time PCR. The possible correlation between the putative lncRNAs and disease severity was also assessed. Receiver operating characteristic (ROC) curve analysis was used to evaluate the predictive role of lncRNAs as diagnostic biomarkers in CAD patients. The expressions of IFNG-AS1 lncRNA as well as IFN-γ and ZEB2 genes were significantly reduced in CAD patients compared to healthy subjects. In contrast, the expression of ZEB2-AS1 was up-regulated in these patients. Linear regression analysis unveiled that there is a positive correlation between the expression of IFNG-AS1 and IFN-γ, also similarly, ZEB2-AS1 and ZEB2 in PBMCs of subjects. Moreover, the expression of IFNG-AS1 and ZEB2-AS1 correlated with the Gensini score. The area under the ROC curves ranged from 0.633–0.742 for ZEB2-AS1/ZEB2 and IFNG-AS1/IFN-γ, respectively.

Conclusions

Our results indicated that the dysregulation of IFNG-AS1/IFN-γ and ZEB2-AS1/ZEB2 in PBMCs of CAD patients may be involved in CAD pathogenesis.

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Acknowledgements

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Authors and Affiliations

  1. Department of Genetics and Advanced Medical Technology, Medical Biotechnology Research Center, Faculty of Medicine, AJA University of Medical Sciences, Tehran, Iran

    Ali rafiei & Farnaz Khodabakhsh

  2. Department of Nanobiotechnology, New Technologies Research Group, Pasteur Institute of Iran, Tehran, Iran

    Reza Ahangari Cohan

  3. Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran

    Fatemeh Azadegan-Dehkordi

  4. Department of Medical Laboratory, Faculty of Paramedical Sciences, Kashan University of Medical Sciences, Kashan, Iran

    Morteza Salimian

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  1. Ali rafiei
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  4. Fatemeh Azadegan-Dehkordi
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This manuscript was approved by all authors.

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Correspondence to Farnaz Khodabakhsh.

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The Ethics Committee of AJA University of Medical Sciences was approved this study under the declaration code of “IR.AJAUMS.REC.1400.140.” All participants signed written informed consent.

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rafiei, A., Khodabakhsh, F., Cohan, R.A. et al. The correlation of long non-coding RNAs IFNG-AS1 and ZEB2-AS1 with IFN-γ and ZEB-2 expression in PBMCs and clinical features of patients with coronary artery disease. Mol Biol Rep 49, 3389–3399 (2022). https://doi.org/10.1007/s11033-022-07168-9

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  • DOI: https://doi.org/10.1007/s11033-022-07168-9

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