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Germin like protein genes exhibit modular expression during salt and drought stress in elite rice cultivars

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Abstract

Background

Germin-like proteins (GLPs) are ubiquitous plant proteins, which play significant role in plant responses against various abiotic stresses. However, the potential functions of GLPs in rice (Oryza sativa) against salt and drought stress are still unclear.

Methods and results

In this study, transcriptional variation of eight OsGLP genes (OsGLP3-6, OsGLP4-1, OsGLP8-4, OsGLP8-7, OsGLP8-10, OsGLP8-11 and OsGLP8-12) was analyzed in leaves and roots of two economically important Indica rice cultivars, KS282 and Super Basmati, under salt and drought stress at early seedling stage. The relative expression analysis from qRT-PCR indicated the highest increase in expression of OsGLP3-6 in leaves and roots of both rice varieties with a significantly higher expression in KS282. Moreover, relative change in expression of OsGLP8-7, OsGLP8-10 and OsGLP8-11 under salt stress and OsGLP8-7 under drought stress was also commonly higher in leaves and roots of KS282 as compared to Super Basmati. Whereas, OsGLP3-7 and OsGLP8-12 after salt stress and OsGLP8-4 and OsGLP8-12 after drought stress were observed with higher relative expression in roots of Super Basmati than KS282. Importantly, the OsGLP3-6 and OsGLP4-1 from chromosome 3 and 4 respectively showed higher expression in leaves whereas most of the OsGLP genes from chromosome 8 exhibited higher expression in roots.

Conclusion

Overall, as a result of this comparative analysis, OsGLP genes showed both general and specific expression profiles depending upon a specific rice variety, stress condition as well as tissue type. These results will increase our understanding of role of OsGLP genes in rice crop and provide useful information for the further in-depth research on their regulatory mechanisms in response to these stress conditions.

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References

  1. Caliskan M (2009) Salt stress causes a shift in the localization pattern of germin gene expression. Genet Mol Res 8(4):1250–1256

    Article  CAS  Google Scholar 

  2. Davidson RM, Reeves PA, Manosalva PM, Leach JE (2009) Germins: a diverse protein family important for crop improvement. Plant Sci 177(6):499–510

    Article  CAS  Google Scholar 

  3. Dunwell JM, Gibbings JG, Mahmood T, Saqlan Naqvi SM (2008) Germin and germin-like proteins: evolution, structure, and function. CRC Crit Rev Plant Sci 27(5):342–375

    Article  CAS  Google Scholar 

  4. Jiang Y, Yang B, Harris NS, Deyholos MK (2007) Comparative proteomic analysis of NaCl stress-responsive proteins in Arabidopsis roots. J Exp Bot 58(13):3591–3607

    Article  CAS  Google Scholar 

  5. Lu M, Han YP, Gao JG, Wang XJ, Bin LW (2010) Identification and analysis of the germin-like gene family in soybean. BMC Genom. https://doi.org/10.1186/1471-2164-11-620

    Article  Google Scholar 

  6. Barman AR, Banerjee J (2015) Versatility of germin-like proteins in their sequences, expressions, and functions. Funct Integr Genomics 15(5):533–548

    Article  CAS  Google Scholar 

  7. Banerjee J, Das N, Dey P, Maiti MK (2010) Transgenically expressed rice germin-like protein1 in tobacco causes hyper-accumulation of H2O2 and reinforcement of the cell wall components. Biochem Biophys Res Commun [Internet] 402(4):637–643. https://doi.org/10.1016/j.bbrc.2010.10.073

    Article  CAS  Google Scholar 

  8. Yasmin T, Mumtaz A, Mahmood T, Hyder MZ, Naqvi SMS (2015) A germin-like protein gene of rice increased superoxide dismutase activity in transformed tobacco. Biol Plant 59(3):456–462

    Article  CAS  Google Scholar 

  9. Li L, Xu X, Chen C, Shen Z (2016) Genome-wide characterization and expression analysis of the germin-like protein family in rice and arabidopsis. Int J Mol Sci 17(10):1622

    Article  Google Scholar 

  10. Banerjee J, Gantait S, Maiti MK (2017) Physiological role of rice germin-like protein 1 (OsGLP1) at early stages of growth and development in indica rice cultivar under salt stress condition. Plant Cell Tissue Organ Cult 131(1):127–137

    Article  CAS  Google Scholar 

  11. Giarola V, Chen P, Dulitz SJ, König M, Manduzio S, Bartels D (2020) The dehydration- and ABA-inducible germin-like protein CpGLP1 from Craterostigma plantagineum has SOD activity and may contribute to cell wall integrity during desiccation. Planta [Internet] 252(5):1–13. https://doi.org/10.1007/s00425-020-03485-0

    Article  CAS  Google Scholar 

  12. Wang L, Shang H, Liu Y, Zheng M, Wu R, Phillips J et al (2009) RESEARCH PAPER A role for a cell wall localized glycine-rich protein in dehydration and rehydration of the resurrection plant Boea hygrometrica. Plant Biol 11:837–848

    Article  CAS  Google Scholar 

  13. Bai XG, Long J, Li S, Li KZ, Xu HN (2014) Molecular cloning and characterisation of a germin-like protein gene in spinach (SoGLP). J Hortic Sci Biotechnol 89(5):592–598

    Article  Google Scholar 

  14. Faghani E, Gharechahi J, Komatsu S, Mirzaei M, Ali Khavarinejad R, Najafi F et al (2015) Data in support of comparative physiology and proteomic analysis of two wheat genotypes contrasting in drought tolerance. Data Brief 2:26–28

    Article  Google Scholar 

  15. Shankar R, Bhattacharjee A, Jain M (2016) Transcriptome analysis in different rice cultivars provides novel insights into desiccation and salinity stress responses. Sci Rep [Internet] 6(March):1–15. https://doi.org/10.1038/srep23719

    Article  CAS  Google Scholar 

  16. Kumar A, Dash PK (2019) Transcriptome analysis for abiotic stresses in rice (Oryza sativa L.). Transcr Anal. 2019;1–16.

  17. Hussain S, Cao X, Zhong C, Zhu L, Khaskheli MA, Fiaz S et al (2018) Sodium chloride stress during early growth stages altered physiological and growth characteristics of rice. Chil J Agric Res 78(2):183–197

    Article  Google Scholar 

  18. Kanawapee N, Sanitchon J, Lontom W, Threerakulpisut P (2012) Evaluation of salt tolerance at the seedling stage in rice genotypes by growth performance, ion accumulation, proline and chlorophyll content. Plant Soil 358(1–2):235–249

    Article  CAS  Google Scholar 

  19. Sumayya K, Rajanna M, Deepak C, Shivakumar K, Denesh G (2020) Evaluation of rice (Oryza sativa L.) genotypes for cold tolerance at seedling stage. Oryza-An Int J Rice 57(1):43–48

    Article  Google Scholar 

  20. Das A, Pramanik K, Sharma R, Gantait S, Banerjee J (2019) In-silico study of biotic and abiotic stress-related transcription factor binding sites in the promoter regions of rice germin-like protein genes. PLoS ONE 14(2):1–22

    Google Scholar 

  21. Ilyas M, Irfan M, Mahmood T, Hussain H, Latif-ur-Rehman NI et al (2019) Analysis of germin-like protein genes (OsGLPs) family in rice using various in silico approaches. Curr Bioinform 15(1):17–33

    Article  Google Scholar 

  22. Walia H, Wilson C, Zeng L, Ismail AM, Condamine P, Close TJ (2007) Genome-wide transcriptional analysis of salinity stressed japonica and indica rice genotypes during panicle initiation stage. Plant Mol Biol 63(5):609–623

    Article  CAS  Google Scholar 

  23. Ijaz B, Formentin E, Ronci B, Locato V, Barizza E, Hyder MZ et al (2019) Salt tolerance in indica rice cell cultures depends on a fine tuning of ROS signalling and homeostasis. PLoS ONE 14(4):1–27

    Article  Google Scholar 

  24. Schmittgen TD, Livak KJ (2008) Analyzing real-time PCR data by the comparative CT method. Nat Protoc 3(6):1101–1108

    Article  CAS  Google Scholar 

  25. Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods 25(4):402–408

    Article  CAS  Google Scholar 

  26. Wani HS (2015) Plant stress tolerance: engineering ABA: a potent phytohormone. Transcr Open Access 03(02):1000113

    Article  Google Scholar 

  27. Wang T, Chen X, Zhu F, Li H, Li L, Yang Q et al (2013) Characterization of peanut germin-like proteins, AhGLPs in plant development and defense. PLoS ONE 8(4):1–11

    Article  Google Scholar 

  28. Wang H, Zhang Y, Xiao N, Zhang G, Wang F, Chen X et al (2020) Rice GERMIN-LIKE PROTEIN 2–1 functions in seed dormancy under the control of abscisic acid and gibberellic acid signaling pathways. Plant Physiol 183(3):1157–1170

    Article  CAS  Google Scholar 

  29. Ham B, Li G, Kang B, Zeng F, Lucas WJ (2012) Overexpression of arabidopsis plasmodesmata germin-like proteins disrupts root growth and development. Plant Cell 24(September):3630–3648

    Article  CAS  Google Scholar 

  30. Sah SK, Reddy KR, Li J (2016) Abscisic acid and abiotic stress tolerance in crop plants. Front Plant Sci 7(MAY2016):1–26

    Google Scholar 

  31. Li Y, Zhang D, Li W, Mallano AI, Zhang Y, Wang T et al (2016) Expression study of soybean germin-like gene family reveals a role of GLP7 gene in various abiotic stress tolerances. Can J Plant Sci 96(2):296–304

    Article  CAS  Google Scholar 

  32. Pei Y, Li X, Zhu Y, Ge X, Sun Y, Liu N et al (2019) Ghabp19, a novel germin-like protein from Gossypium hirsutum, plays an important role in the regulation of resistance to verticillium and fusarium wilt pathogens. Front Plant Sci 10(May):1–18

    CAS  Google Scholar 

  33. Fatehi F, Hosseinzadeh A, Alizadeh H, Brimavandi T, Struik PC (2012) The proteome response of salt-resistant and salt-sensitive barley genotypes to long-term salinity stress. Mol Biol Rep 39(5):6387–6397

    Article  CAS  Google Scholar 

  34. Kamal AHM, Cho K, Kim DE, Uozumi N, Chung KY, Lee SY et al (2012) Changes in physiology and protein abundance in salt-stressed wheat chloroplasts. Mol Biol Rep 39(9):9059–9074

    Article  CAS  Google Scholar 

  35. Javed T, Shabbir R, Ali A, Afzal I, Zaheer U, Gao SJ (2020) Transcription factors in plant stress responses: challenges and potential for sugarcane improvement. Plants 9(4):1–18

    Article  Google Scholar 

  36. Tsuda K, Somssich IE (2015) Transcriptional networks in plant immunity. New Phytol 206(3):932–947

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors thank National Agricultural Research Council; Islamabad, Pakistan for providing certified seeds of selected rice varieties. The authors acknowledge the research support provided by the Department of Biology, University of Copenhagen, Denmark. The authors are grateful to Dr Humaira Shaheen, Department of Biosciences, CUI, Islamabad, Pakistan and Mr Ayaz Shahid, Department of Marketing, Linnaeus University, Vaxjo, Sweden for their assistance in statistical analysis.

Funding

This study was funded by International Research Support Initiative Programme (IRSIP) (No. 1-8/HEC/HRD/2018/8554); Higher Education Commission, Pakistan and Research Grant Program (No. 16-58/CRGP/CUI/ISB/18/682); COMSATS University Islamabad, Pakistan awarded to Ms Jazba Anum.

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Authors and Affiliations

  1. Department of Biosciences, COMSATS University Islamabad, Islamabad Campus, Park Road, Islamabad, Pakistan

    Jazba Anum, M. Zeeshan Hyder, Sumaira Farrukh & Tayyaba Yasmin

  2. Department of Biology, University of Copenhagen, 5 Ole Maaloes Vej, Copenhagen, Denmark

    Charlotte O’Shea & Karen Skriver

  3. Department of Plant Breeding and Genetics, Faculty of Crop and Food Sciences, PMAS Arid Agriculture University Rawalpindi, Rawalpindi, 46300, Pakistan

    Saad Imran Malik

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  1. Jazba Anum
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  2. Charlotte O’Shea
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  3. M. Zeeshan Hyder
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  4. Sumaira Farrukh
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  5. Karen Skriver
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  6. Saad Imran Malik
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  7. Tayyaba Yasmin
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Contributions

Conceptualization: [JA], [TY]; Data Curation: [JA]; Methodology: [CO], [JA], [TY]; Formal analysis: [JA], [MZH], [SF], [SIM]; Investigation: [CO], [JA]; Validation: [MZH], [TY]; Writing—original draft preparation: [JA]; Writing—review and editing: [SF], [SIM], [TY]; Funding acquisition: [JA]; Resources: [KS]; Project Administration: [CO], [JA], [KS]; Supervision: [KS], [MZH], [TY].

Corresponding author

Correspondence to Tayyaba Yasmin.

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Anum, J., O’Shea, C., Zeeshan Hyder, M. et al. Germin like protein genes exhibit modular expression during salt and drought stress in elite rice cultivars. Mol Biol Rep 49, 293–302 (2022). https://doi.org/10.1007/s11033-021-06871-3

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  • DOI: https://doi.org/10.1007/s11033-021-06871-3

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