Abstract
Background
Pediatric bronchial asthma signifies a frequent chronic inflammatory airway disorder influencing many children. Despite its irrefutable importance, its exact pathogenesis is not completely elucidated.
Aim of the Study
The study aimed to investigate the correlation between mitophagy machinery proteins, ER stress biomarkers and total polyamine and their role in disease progression via targeting NF-κB mechanisms.
Methods
Sixty children with atopic bronchial asthma were enrolled in the study, they were allocated into 2 equal groups (mild/moderate and severe atopic asthmatic groups). Thirty age-matched healthy control subjects were also included in the study to represent the control group. Phosphatase and tensin homolog (PTEN)-induced kinase-1 (PINK-1) and Parkin messenger RNA (mRNA) expressions were assessed by (RT‐PCR) technique. Levels of inositol requiring enzyme 1α (IRE1α), total polyamines, interleukin 6 & 8 (IL-6, IL-8) and nuclear factor kappa B (NF-κB) were assessed by enzyme‐linked immunosorbent assay. Oxidative stress (OS) biomarkers were also measured.
Results
PINK-1 and PARK mRNA expressions were significantly upregulated in asthmatic patients. Likewise, the level of IRE1α, total polyamines, inflammatory cytokines, and OS biomarkers were significantly elevated in asthmatic groups comparing to control group with the highest levels noticed in severe atopic asthmatic group.
Conclusion
the study documented a correlation between mitophagy machinery proteins, ER stress biomarkers and total polyamines that may pave a new platform to understand pediatric asthma pathogenesis and could be used as reliable biomarkers to evaluate disease progression.
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Data availability
The datasets generated during and/or analyzed during the current study are not publicly available but are available from the corresponding author on reasonable request.
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This research work is performed by using the author's personal fund i.e. the research work is funded by the authors themselves.
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A. K. Eltokhy, designed the study and did the experimental work. O. Toema, examined the children and classify the groups. O. S. Eldeeb, collected the data and recorded the results. All the team shared in the writing and revision of the article.
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Before being enrolled in the current study, all subjects` parents gave a written informed consents for participation and publication. The study plan was approved by the Local Research Ethics Committee of the Faculty of Medicine, Tanta University (Approval Code 33884/6/20), and was in accordance with the principles of the Declaration of Helsinki II.
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Eltokhy, A.K., Toema, O. & El-Deeb, O.S. The Correlation Between PINK-1/Parkin Mediated Mitophagy, Endoplasmic Reticulum Stress and Total Polyamines in Pediatric Bronchial Asthma: An Integrated Network of Pathways. Mol Biol Rep 49, 227–235 (2022). https://doi.org/10.1007/s11033-021-06861-5
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DOI: https://doi.org/10.1007/s11033-021-06861-5