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Investigation of the relationship between MMP-1 (− 1607 1G/2G), MMP-3 (− 1171 5A/6A) gene variations and development of bladder cancer

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Abstract

Background

Chronic inflammation is an important risk factor in the development of bladder cancer. It may stimulate growth and metastasis of cancer cells. The inflammatory process includes MMP activities and expression. MMP activation can be stimulated by various inflammatory cells. Pathological processes such as bladder cancer may occur due to imbalance in MMP activities. In our study, we aimed to determine the relationship between MMP-1, MMP-3 gene variations associated with chronic inflammation and the bladder cancer development.

Methods

Our study was carried out with 89 bladder cancer patients and 78 healthy controls. PCR–RFLP methods were applied to determine MMP-1 and MMP-3 gene variations genotype distributions.

Results

1G/1G homozygous and 1G/2G heterozygous genotypes of MMP-1 gene variation were determined more in patients than controls. The 5A/5A homozygous and 5A/6A heterozygous genotypes of the MMP-3 gene variation were detected more in patients than controls. The significant difference was detected in terms of genotype distributions of MMP-1 and MMP-3 gene variations between these groups (p < 0.05). In addition to, the most common haplotype in the patient group were detected as 1G/2G-5A/6A (20.22%).

Conclusion

In this study, MMP-1 and MMP-3 gene variations were determined as possible genetic risk factors for bladder cancer development in the Thrace population.

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Acknowledgements

This study was performed at departments of Biophysics and Urology in Trakya University Faculty of Medicine. In our study, we would like to thanks at Department of Biostatistics and Medical Informatics in Haliç University Faculty of Medicine.

Funding

This study was not supported by any specific grant from funding.

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Authors and Affiliations

  1. Department of Biophysics, Faculty of Medicine, Trakya University, 22030, Edirne, Turkey

    Arzu Ay

  2. Department of Biophysics, Faculty of Medicine, Haliç University, 34445, Istanbul, Turkey

    Nevra Alkanli

  3. Department of Urology, Faculty of Medicine, Trakya University, 22030, Edirne, Turkey

    Gokhan Cevik

Authors
  1. Arzu Ay
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  2. Nevra Alkanli
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  3. Gokhan Cevik
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by [AA], [NA] and [GC]. The first draft of the manuscript was written by [AA and NA] and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript. The corresponding author attests that all listed authors meet the authorship criteria and that no other authors meeting the criteria have been omitted.

Corresponding author

Correspondence to Arzu Ay.

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Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

For our study, ethics committee approval was obtained with the TÜTF-BAEK 2020/127 protocol code from Trakya University Faculty of Medicine Non-Invasive Clinical Research Ethics Committee.

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Signed informed consent form was collected from each individual in the patient group with bladder cancer and healthy control group.

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Ay, A., Alkanli, N. & Cevik, G. Investigation of the relationship between MMP-1 (− 1607 1G/2G), MMP-3 (− 1171 5A/6A) gene variations and development of bladder cancer. Mol Biol Rep 48, 7689–7695 (2021). https://doi.org/10.1007/s11033-021-06775-2

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  • DOI: https://doi.org/10.1007/s11033-021-06775-2

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