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Detection of hypermethylation BRCA1/2 gene promoter in breast tumours among Moroccan women

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Abstract

Background

The promoter region is a key element of gene expression regulation. In mammals, most of the genes present, at the level of their promoter, a large number of islands CpG. Age also is seen as another factor for developing breast cell cancer reaching the tumour stage.

Aim

This study aimed to explore the hypermethylation of the BRCA1/2 promoter gene in women breast cancer and correlation with age and tumour stage.

Materials and methods

Fifty biopsies were derived from Moroccan women treated for breast carcinoma, the DNA extracted was treated by bisulphite and the targeted BRCA1/2 Amplicons were amplified by specific methylation primers (MSP).

Results

The result shows that 62% of the samples were BRCA1 methylated in addition and negative result for BRCA2, these positive epigenetic factor were remarkable in women over 47 years and at the stage of malignant tumour.

Conclusion

These results show that half of the methylated samples are positive with a majority of over 47 years old, and confirms that age might be an additional factor for breast cancer development.

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Data availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request. The project respects the agreement of the ethics committee (No. 3/2018/30 April/2018-Morocco).

Abbreviations

BRCA1 :

Breast cancer 1

BRCA2 :

Breast cancer 2

CpG :

Cytosine-phosphate-guanine

DNA :

Disoxyribonucleotide

CGI :

Cytosine-guanine island

Bp :

Base pairs

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Acknowledgements

We would like first to express our gratitude to Hassan II University for the provided financial support of this work, we are also grateful to the biopsies donors and physicians and contributors to this study. Our gratitude goes also to the members of the team of virology, oncology, medical and laboratory of virology microbiology quality biotechnologies / ecotoxicology biodiversity at the Faculty of Sciences and Technics-Mohammedia, University Hassan II of Casablanca, the CNRST—Rabat and the Ministry of Higher Education of the kingdom of Morocco for their support for this research.

Funding

This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

Author information

Authors and Affiliations

  1. Team of Virology, Oncology and Medical Biotechnologies, Laboratory of Virology, Microbiology, Quality and Biotechnologies/ETB, Faculty of Sciences and Technics–Mohammedia, University Hassan II of Casablanca, Po Box 146, Mohammedia, Morocco

    Imane Saif, Amal Bouziyane, Mustapha Benhessou & Moulay Mustapha Ennaji

  2. Department of Gynecological Obstetrics, University Mohamed VI of Health Sciences of Casablanca, Casablanca, Morocco

    Amal Bouziyane

  3. Department of Gynecological Obstetrics, Faculty of Medicine of Casablanca, Hospital University Center (CHU) Ibn Rochd Casablanca, University Hassan II, Casablanca, Morocco

    Mustapha Benhessou & Mohamed El Karroumi

Authors
  1. Imane Saif
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  2. Amal Bouziyane
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  3. Mustapha Benhessou
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  4. Mohamed El Karroumi
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  5. Moulay Mustapha Ennaji
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Contributions

Supervision-MME, AB, MB, MEK; Materials- MME; Data collection&/or processing-MME, AB, MB, MEK; Analysis and or interpretation-MME; literature search-MME; writing-MME.

Corresponding author

Correspondence to Moulay Mustapha Ennaji.

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Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

The study was approved by the Biomedical Research Ethics Committee of the School of Medicine and Pharmacy of Casablanca.

Consent to participate

All patients consented to participate in the study according to the ethical standards.

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All authors approved and consented to publish the presented results.

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Saif, I., Bouziyane, A., Benhessou, M. et al. Detection of hypermethylation BRCA1/2 gene promoter in breast tumours among Moroccan women. Mol Biol Rep 48, 7147–7152 (2021). https://doi.org/10.1007/s11033-021-06705-2

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  • DOI: https://doi.org/10.1007/s11033-021-06705-2

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