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Development of the first microsatellite markers using high-throughput sequencing for a hexaploid coastal species, Tournefortia argentea L. f. (Boraginaceae)

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Abstract

Backgroud

Tournefortia argentea L. f. is a hexaploid shrub or tree species with ecological and evolutionary significances, which forms the fringe of vegetation closest to the sea on tropical coral islands. Previous studies have never addressed on genetic information, and thus genomic resources remain scarce.

Methods and results

We used nine individuals from different islands to identify polymorphic microsatellites of T. argentea by Illumina high-throughput sequencing. Thirty-five polymorphic microsatellite markers were developed. Characteristics of each locus were tested using 48 individuals collected from three populations of T. argentea. A total of 320 alleles were found across the 35 microsatellite loci. The number of alleles per locus ranged from 5 to 15, with an average of 9.1. Observed and expected heterozygosities in each locus per population varied from 0.000 to 1.000 and from 0.000 to 0.893, respectively.

Conclusions

In this study, we report the development of 35 polymorphic microsatellite markers based on Illumina high-throughput sequencing. These markers will facilitate the investigations of genetic diversity, population structures and evolutionary history of T. argentea.

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Fig. 1

Data availability

Sequence information for the developed primers has been deposited in the National Center for Biotechnology Information (NCBI), and GenBank accession numbers are provided in Table 1.

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Acknowledgements

We thank Xinxin Zhou and Guowang Tang for sample collections. This work was supported by the National Natural Science Foundation of China (Grant No. 32170232) and Biological Resources Program, Chinese Academy of Sciences (Grant No. KFJ-BRP-017-35).

Funding

This work was supported by the National Natural Science Foundation of China (Grant No. 32170232) and Biological Resources Program, Chinese Academy of Sciences (Grant No. KFJ-BRP-017-35).

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Authors and Affiliations

  1. Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China

    Miaomiao Shi, Tieyao Tu & Dianxiang Zhang

  2. National Demonstration Center for Experimental Plant Science Education, College of Agriculture, Guangxi University, Nanning, China

    Qiubiao Zeng

Authors
  1. Miaomiao Shi
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  2. Qiubiao Zeng
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  3. Tieyao Tu
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  4. Dianxiang Zhang
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Contributions

MS conducted the lab work, analyzed the data and wrote the manuscript. QZ collected the samples. TT and DZ contributed to conceptualization and revision of the manuscript.

Corresponding author

Correspondence to Dianxiang Zhang.

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Shi, M., Zeng, Q., Tu, T. et al. Development of the first microsatellite markers using high-throughput sequencing for a hexaploid coastal species, Tournefortia argentea L. f. (Boraginaceae). Mol Biol Rep 48, 7049–7055 (2021). https://doi.org/10.1007/s11033-021-06668-4

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