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Human adipose derived stem cell exosomes enhance the neural differentiation of PC12 cells

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Abstract

Human adipose stem cells (hADSCs) are proper cell sources for tissue regeneration. They mainly mediate their therapeutic effects through paracrine factors as exosomes. The exosomes contents are protein, lipid and RNA. Exosomes are effective in restoring the function of neurons and astrocytes in neurodegenerative diseases, and improve the therapeutic outcomes. We investigated the effect of hADSCs derived exosomes on survival and neural differentiation of PC12 cells in vitro. The isolated hADSCs, were characterized by flow cytometry. Exosomes were separated from hADSC-condition medium using Exo-spinTM kit and characterized by DLS and TEM. Then acridine orange staining was performed to confirm entrance of exosomes into PC12 cells. PC12 cells were treated with culture medium containing NGF and exosome. Cell viability was assessed by MTT assay, and neural differentiation by ICC technique and qRT-PCR. TEM and DLS data confirmed the isolation of exosomes according to their size (30–100 nm) and acridine orange staining indicated entrance of exosomes to target cells. MTT assay showed that cell viability was significantly increased in exosome treated group. ICC technique revealed that the expression of Map2 was superior in the exosome treated group. Based on qRT-PCR data, Map2 and β-tub III gene expression was increased in the exosome treated group. Significant expression of Gfap was seen in the NGF and NGF/EXO treated groups. Present study indicated that hADSCs derived exosomes might enhance cell viability and promote neuronal differentiation and expression of mature neural marker in PC12 cells.

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Acknowledgements

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Funding

This study extracted from a M.Sc project and financially supported by Isfahan University of Medical Sciences (Project No. 396571).

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Authors and Affiliations

  1. Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

    Samira Shariati Najafabadi, Noushin Amirpour, Sharhram Amini & Hossein Salehi

  2. Applied Physiology Research Center, Cardiovascular Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran

    Nasrin Zare

  3. Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

    Mohammad Kazemi

Authors
  1. Samira Shariati Najafabadi
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  2. Noushin Amirpour
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  3. Sharhram Amini
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  4. Nasrin Zare
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  5. Mohammad Kazemi
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  6. Hossein Salehi
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by Samira Shariati Najafabadi, Noushin Amirpour and Hossein Salehi. The first draft of the manuscript was written by Samira Shariati Najafabadi and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Hossein Salehi.

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Conflict of interest

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Informed consent

The adipose tissue samples were legally achieved by collecting a written consent approved by Care Committee of Isfahan University of Medical Sciences (IR.MUI.REC.1396.3.571).

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Shariati Najafabadi, S., Amirpour, N., Amini, S. et al. Human adipose derived stem cell exosomes enhance the neural differentiation of PC12 cells. Mol Biol Rep 48, 5033–5043 (2021). https://doi.org/10.1007/s11033-021-06497-5

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  • DOI: https://doi.org/10.1007/s11033-021-06497-5

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