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Correlation of perilipin 2 and lipid metabolism in elective cesarean section and vaginal delivery: a prospective study with oxidative and apoptotic pathways

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Abstract

Vaginal delivery (VD) and elective cesarean (CS) delivery modes may cause significant differences in maternal and fetal metabolism. In this study, we aimed to investigate changes in lipid metabolism, oxidative and apoptotic signaling pathways during VD and CS in maternal and cord blood and placenta tissue. The study included two groups of participants delivered via 90 CS and 90 VD. Maternal and cord blood samples were collected from the participants. In addition, placenta samples were also taken after delivery. Total oxidant (TOS), malondialdehyde (MDA), total antioxidant (TAS), glutathione (GSH), cleaved caspase 3 (CASP3) and perilipin 2 (PLIN2) levels were measured to determine oxidative stress, antioxidant levels and apoptosis status in the VD and CS groups. Besides, PLIN2 mRNA expressions in placental specimens were analyzed. We found no statistically significant difference in maternal age, body mass index, gestational age, birth weight and Apgar scores in both groups (P > 0.05). The increase in MDA, TOS, GSH and TAS levels was higher in the VD group compared to the CS group (P < 0.05). Similarly, PLIN2 levels and lipid profiles showed an increase in the VD group (P < 0.05 vs CS group). Likewise, PLIN2 expression enhanced in the VD group (P < 0.05 vs CS group). However, CASP3 activity reduced in maternal and cord blood in the VD group compared to the CS group. Our results support that the delivery mode may cause differences in lipid profile, oxidative and apoptotic status by affecting PLIN2 levels in both maternal and cord blood and placenta tissue.

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Authors and Affiliations

  1. Department of Medical Biochemistry, Faculty of Medicine, Düzce University, Duzce, Turkey

    Ceyhan Hacioglu & Ibrahim Ethem Sahin

  2. Department of Obstetrics and Gynecology, Faculty of Medicine, Düzce University, Duzce, Turkey

    Can Uyuk

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  1. Ceyhan Hacioglu
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Contributions

CH and IES contributed to the study conception and design. Material preparation, data collection and analysis were performed by CH, IES and CU. The first draft of the manuscript was written by CH and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Ceyhan Hacioglu.

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The authors declare that they have no conflict of interest.

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The study protocol was approved by the Düzce University Non-Invasive Health Research Ethics Committee (Decision no: 2020/30) and was carried out in accordance with the Helsinki declaration.

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Informed consent was obtained from all individual participants included in the study.

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Hacioglu, C., Sahin, I.E. & Uyuk, C. Correlation of perilipin 2 and lipid metabolism in elective cesarean section and vaginal delivery: a prospective study with oxidative and apoptotic pathways. Mol Biol Rep 48, 3991–3998 (2021). https://doi.org/10.1007/s11033-021-06399-6

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  • DOI: https://doi.org/10.1007/s11033-021-06399-6

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