Abstract
Introduction
Oxidative stress is closely related to the pathophysiology of gestation, where the placenta is susceptible to oxidative damage, contributing to the onset of gestational complications. Currently, few studies evaluate the use of oxidative markers for prediction of risk of gestational complications. However, there are some reports that suggest these biomarkers as potential prognostic biomarkers. Therefore, the objective of this study was to compare the biomarkers of oxidative stress from gestations with and without complications, and also evaluate the delta of variation in these markers from the first gestational trimester.
Material and Methods
A total of 45 pregnant women were evaluated during the three gestational trimesters, of whom 15 developed gestational complications by the end of gestation. The evaluated oxidative damage markers were thiobarbituric acid reactive substances and nitric oxide dosage. Evaluation of the antioxidant system was performed by the quantification of vitamin C, sulfhydryl groups, total antioxidant capacity, plasmatic iron reduction ability, the evaluation of catalase and delta-aminolevulinate dehydratase enzymatic activity.
Results
According to the results, the markers of oxidative damage are increased, and the antioxidant profile decreased, in the third trimester of complicated pregnancies as compared to uncomplicated pregnancies. Moreover, the delta of variation in both oxidative damage markers and antioxidants was higher in complicated gestations as compared to uncomplicated gestations, thus suggesting a higher oxidative stress in pregnancies with complications.
Conclusions
Oxidative stress parameters appear altered in pregnant women with gestational complications. The markers to oxidative stress can be possible biomarkers, helping in understanding mechanisms underlying the associations between complications during pregnancy and various health outcomes.
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Abbreviations
- CAT:
-
Catalase
- δ-ALA-D:
-
Delta-aminolevulinate dehydratase
- DTT:
-
Dithiothreitol
- MDA:
-
Malondialdehyde
- NOx:
-
Nitric oxide
- FRAP:
-
Plasmatic iron reduction ability
- ROS:
-
Reactive oxygen species
- TBA:
-
Thiobarbituric acid
- TBARS:
-
Thiobarbituric acid reactive substances
- SH:
-
Thiol groupings
- TAC:
-
Total Antioxidant capacity
- VIT C:
-
Vitamin C
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Acknowledgements
The authors thank all the volunteers who participated in this study. Also, we thank Fundação Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Fundo de Incentivo à Pesquisa (FIPE), municipal health secretariat of Santa Maria, Federal University of Santa Maria (UFSM) and the University Hospital of Santa Maria (HUSM), Rio Grande do Sul, Brazil, for support in this study.
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This study was approved by the Department of Education, Research and Extension (DEPE) of the University Hospital of Santa Maria, later by the Research Ethics Committee (CEP) of the Federal University of Santa Maria (UFSM), received the Certificate of Presentation for Ethical Appreciation (CAAE) under number: 62643616.2.0000.5346.
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A total of 57 pregnant women agreed to participate in the study, after accepting the Free and Informed Consent Form (TCLE), which was read and signed by all participants.
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de Lucca, L., Jantsch, L.B., Vendrame, S.A. et al. Variation of the Oxidative Profile in Pregnant Women With and Without Gestational Complications. Matern Child Health J 26, 2155–2168 (2022). https://doi.org/10.1007/s10995-022-03475-6
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DOI: https://doi.org/10.1007/s10995-022-03475-6