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Two different begomovirus species are associated with yellow vein mosaic disease of okra in Sri Lanka

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Abstract

Yellow vein mosaic disease is the major biotic constraint of okra cultivation in Sri Lanka. Identification and detailed molecular characterization of associated pathogen is needed for effective disease management. The genome of the begomovirus and betasatellite were amplified in symptomatic plant samples using specific degenerate primers. DNA-A genome of twelve isolates representing different locations in Sri Lanka were cloned, sequenced and deposited in GenBank database (Accession No- KX698087- KX698092 and MH455207- MH455212). Size of the complete nucleotide sequences ranged from 2735 to 2786 bp. The genome organization showed characteristics of begomoviruses. The pairwise sequence identity revealed the association of two different begomovirus species. Five of the isolates showed > 91% of sequences identity with Bhendi yellow vein mosaic virus, and the rest of the seven isolates were around 92% of identity with Okra enation leaf curl virus. This is further supported by phylogenetic analysis where both of these group of isolates were in different cluster. Recombination analysis showed the presence of recombinant fragments in the virus isolates associated with okra yellow vein mosaic disease (OYVMD) in Sri Lanka. Attempts to amplify DNA- B were failed in any of the samples tested. However, both type of the begomovirus species associated with betasatellite species, Bhendi yellow vein mosaic betasatellite. The present study has revealed the association of two distinct monopartite begomovirus species, Bhendi yellow vein mosaic virus or Okra enation leaf curl virus, with OYVMD in Sri Lanka.

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Acknowledgements

The authors are grateful to Dr. George Gibbings and Dr. Ihsan Ullah (Molecular Genetics Laboratory, School of Agriculture, Policy and Development, University of Reading, UK) for technical assistance.

Funding

The works in Sri Lanka was supported by University Grants Commission, Government of Sri Lanka under the project: UGC/DRIC/PG/2015(ii)/JFN/01.

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Authors and Affiliations

  1. Department of Botany, Faculty of Science, University of Jaffna, Jaffna, Sri Lanka

    T. C. Jeyaseelan

  2. Department of Agricultural Biology, Faculty of Agriculture, University of Peradeniya, Peradeniya, Sri Lanka

    D. M. De Costa

  3. School of Agriculture, Policy and Development, University of Reading, Reading, UK

    M. W. Shaw

  4. Plant Protection Board of Study, Postgraduate Institute of Agriculture, University of Peradeniya, Peradeniya, Sri Lanka

    T. C. Jeyaseelan & D. M. De Costa

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  1. T. C. Jeyaseelan
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  3. M. W. Shaw
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Contributions

This work was carried out in collaboration between all authors. TCJ contributed designing the experiments, carried out all experiments and wrote the first draft of the manuscript. DMDe C designed and supervised the experimental process. MWS performed data analysis and supervised the experiments.. All authors read and approved the final manuscript.

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Correspondence to T. C. Jeyaseelan.

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Jeyaseelan, T.C., De Costa, D.M. & Shaw, M.W. Two different begomovirus species are associated with yellow vein mosaic disease of okra in Sri Lanka. Mol Biol Rep 48, 1383–1391 (2021). https://doi.org/10.1007/s11033-021-06213-3

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