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Preadministration of yerba mate (Ilex paraguariensis) helps functional activity and morphology maintenance of MC3T3-E1 osteoblastic cells after in vitro exposition to hydrogen peroxide

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Abstract

Natural substances with antioxidant effects may benefit prevention and treatment of people with or prone to bone diseases after menopause, such as osteoporosis. This study aimed to evaluate the in vitro effect of preadministration of yerba mate extract (YM) in the metabolism of MC3T3-E1 osteoblasts exposed to hydrogen peroxide (H2O2). The cells (MC3T3-E1) were cultured in 24-well plates with the concentration of 1 μg/mL yerba mate extract dissolved in culture medium throughout the culture period. Four hours before each experiment, 400 μmol/L H2O2 was added per well to simulate oxidative stress. There were evaluated cell adhesion and proliferation, in situ detection of alkaline phosphatase (ALP), mineralized nodules, and immunolocalization of osteocalcin (OCN), bone sialoprotein (BSP) and alkaline phosphatase (ALP) proteins. The results showed that YM preadministration to H2O2 exposition significatively increased cell adhesion after 3 days as well as proliferation and in situ ALP detection after 10 and 7 days respectively, when compared to H2O2 group. Besides, staining of OCN and BSP proteins was less intense and scattered in poor spread cells with cytoskeletal changes in H2O2 group when compared to control and YM H2O2 group. ALP staining was restrained to intracellular regions and similar in all experimental groups. Our results suggest that preadministration of yerba mate extract may prevent deleterious effects in the morphology and functional activity of osteoblasts exposed to H2O2, which could enable the maintenance of extracellular matrix in the presence of oxidative stress.

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Acknowledgements

We thank São Paulo Research Foundation (FAPESP) and National Institute of Science and Technology, Translational Medicine (Brazil) for supporting the project research.

Funding

This research was supported by the São Paulo Research Foundation (FAPESP 2017/24679-3) and by National Institute of Science and Technology, Translational Medicine, Brazil.

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Authors and Affiliations

  1. Department of Basic and Oral Biology, Bone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil

    Gabrielli Collasanto Ceverino, Guilherme Alvarenga Alves, José Bernardo de Santis, Selma Siéssere & Karina Fittipaldi Bombonato-Prado

  2. Unit of Basic Oral Investigation-UIBO, School of Dentistry, Universidad El Bosque, Bogotá, Colombia

    Paula Katherine Vargas Sanchez

  3. Department of Oral and Maxillofacial Surgery and Periodontology, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil

    Roger Rodrigo Fernandes & Milla Sprone Tavares

Authors
  1. Gabrielli Collasanto Ceverino
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  2. Paula Katherine Vargas Sanchez
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  3. Roger Rodrigo Fernandes
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  4. Guilherme Alvarenga Alves
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  5. José Bernardo de Santis
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  7. Selma Siéssere
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  8. Karina Fittipaldi Bombonato-Prado
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Contributions

GCC received the FAPESP scholarship to perform the project. She performed all the experiments and helped with manuscript writing. PKVS helped to plan the experiments and in cell line proliferation to be used the all experimental groups. RRF is the cell culture lab technician and supervised all the experiments performed by the students. GAA helped with the cell culture and biochemical assays. JBS helped with data interpretation and statistical analysis. MST helped with biochemical assays. SS helped with data analysis and manuscript writing. KFBP idealized the project, supervised the experiments, analyzed the results and wrote the manuscript.

Corresponding author

Correspondence to Karina Fittipaldi Bombonato-Prado.

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The authors declare they have no conflicts/competing of interest.

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Ethical approval was not required because the experiments utilized MC3T3-E1 cell line.

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Ceverino, G.C., Sanchez, P.K.V., Fernandes, R.R. et al. Preadministration of yerba mate (Ilex paraguariensis) helps functional activity and morphology maintenance of MC3T3-E1 osteoblastic cells after in vitro exposition to hydrogen peroxide. Mol Biol Rep 48, 13–20 (2021). https://doi.org/10.1007/s11033-020-06096-w

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  • DOI: https://doi.org/10.1007/s11033-020-06096-w

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