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RAPD-based evaluation of genetic diversity among populations of the Iranian endemic species Rhabdosciadium aucheri Boiss. (Apiaceae)

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Abstract

Rhabosciadium aucheri is an Iranian endemic herbaceous species that grows in the west, center, and south regions of Iran. In the present study, genetic variation of 70 individuals belonging to seven natural populations of four provinces was investigated using Random Amplified Polymorphic DNA (RAPD) markers. Ten out of twenty-two RAPD primers employed in this study, generated 110 highly amplified and reproducible loci and a mean of 11.1 bands per primer and 48.13% of polymorphism was obtained. According to our results, the primer OPA10 presented the highest effective number of alleles, Shannon’s index, and genetic diversity. The highest value of genetic identity (0.916) was determined between Hamadan, Nahavand and Hamadan, Alvand Mts. populations and the highest genetic distance (0.277) was observed between Hamadan, Asadabad and Kurdistan, Qorveh populations. Therefore, there is an obvious correlation between genetic diversity and geographical distribution. PCA was obtained based on RAPD molecular data and Neighbor Joining (NJ) dendrogram was provided successively. Similar results were attained employing UPGMA and Neighbor Joining dendrograms, supported by PCA ordination plot. Overall, almost moderate level of polymorphism was obtained by RAPD molecular markers at the population level that shows a reasonable amount of intraspecific variability. The reason might be due to the low level of gene flow between populations that could give rise to high genetic differentiation.

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Acknowledgements

The authors gratefully acknowledge the financial supports of the Islamic Azad University, Science and Research Branch (Tehran, Iran). The authors also wish to thank all laboratory staffs for providing equipment to accomplish this research and Mr. A. Sadeghi for improving the quality of English language of the article.

Funding

This work was supported by the Islamic Azad University, Science and Research Branch, Tehran, Iran.

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Authors and Affiliations

  1. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Fatemeh Kazemeini & Iraj Mehregan

  2. Department of Botany, Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran

    Younes Asri

  3. Department of Biology, Yadegar-e-Imam Khomeini (RAH) Shahre Rey Branch, Islamic Azad University, Tehran, Iran

    Golaleh Mostafavi

  4. Natural Resources Research Division, Hamadan Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Hamadan, Iran

    Ramezan Kalvandi

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  1. Fatemeh Kazemeini
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Correspondence to Golaleh Mostafavi.

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On behalf of all authors, the corresponding author declare that, permission to collect an endemic and rare species was not required in Iran.

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Kazemeini, F., Asri, Y., Mostafavi, G. et al. RAPD-based evaluation of genetic diversity among populations of the Iranian endemic species Rhabdosciadium aucheri Boiss. (Apiaceae). Mol Biol Rep 47, 9345–9352 (2020). https://doi.org/10.1007/s11033-020-06028-8

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