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Alterations in niban gene expression as a response to stress conditions in 3T3-L1 adipocytes

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Abstract

Adipocyte death is important in obesity development. Understanding and prevention of adipocyte deaths may be a molecular approach in the treatment. In the study, we aimed to understand role of Niban gene, which acts as an anti-apoptotic molecule as a response to stress conditions, in adipocytes. 3T3-L1 adipocytes were treated with different doses of linoleic acid, hydrogen peroxide and ethanol; and proliferation of the cells examined with real time monitoring iCELLingence system. Gene expression levels were measured by q-PCR. As a response to 24h 480 µM linoleic acid treatment, Niban gene expression was found to be higher than control group (p = 0.008), whereas 24 h 90 mM ethanol treatment was determined to be lower than control group (p = 0.008). The highest value of Niban gene expression among H2O2 treatment groups was detected in 4h 600µM H2O2 in comparison to control group (p = 0.008). To understand role of Niban in adipogenesis, Niban gene expressions were compared between pre-adipocytes and advanced fat accumulated adipocytes and determined to be significantly different (p = 0.042). Our results suggest that Niban might be involved in stress response process in adipocytes. However, the exact molecular role of Niban needs to be investigated in further studies.

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Acknowledgements

This study was supported by Marmara University Scientific Research Projects Coordination Unit. Project No: FEN-C-YLP-080415-0125. Additionally, we would like to thank the Scientific and Technological Research Council of Turkey (TUBITAK) for supporting education of young scientists (M. Cevik) with 2210-C master program.

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Authors and Affiliations

  1. Faculty of Arts and Sciences, Division of Biology, Department of Molecular Biology, Marmara University, Istanbul, Turkey

    Mehtap Cevik & Belgin Susleyici

  2. Department of Medical Services and Techniques, Kutahya Health Sciences University, Kutahya, Turkey

    Meliha Koldemir Gunduz

  3. Faculty of Engineering and Architecture, Department of Software Engineering, Beykoz University, Istanbul, Turkey

    Gokce Deliorman

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  1. Mehtap Cevik
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Cevik, M., Gunduz, M.K., Deliorman, G. et al. Alterations in niban gene expression as a response to stress conditions in 3T3-L1 adipocytes. Mol Biol Rep 47, 9399–9408 (2020). https://doi.org/10.1007/s11033-020-05992-5

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