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Mediation of induced systemic resistance by the plant growth-promoting rhizobacteria Bacillus pumilus S2-3-2

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Abstract

Plant-rhizobacteria interaction and co-evolution developed adaptive strategies which may help the plant survive in nature. Plant rhizosphere soil isolates were analyzed to investigated the effects of rhizobacteria for promoting plant growth and suppress plant disease. Bacterial strains which isolated from plant rhizosphere soil were screened for elicitation of induced systemic resistance (ISR) on tobacco. Strain S2-3-2 results in significant reduction of disease severity on tobacco, it was identified as Bacillus pumilus by multilocus sequence analysis (MLSA). Strain S2-3-2 was deeper studied for pepper plant growth promotion and biological control activity against pepper bacterial spot disease. It was found that the pepper disease severity was decreased when the roots were drenched with strain S2-3-2, and the pepper plants had a higher weight and chlorophyll content, as compared with the mock-treated plants. Transcriptional expression of pathogenesis-related (PR) protein genes in pepper was analyzed by real-time PCR, gene expressions of CaPR1, CaPR4, and CaPR10 were increased when the plants were treated with strain S2-3-2. Moreover, strain S2-3-2 was tested for the production of indole-3-acetic acid (IAA), and it was determined to emit volatiles that enhance the growth of the tobacco plants. Interesting, heat-killed S2-3-2 enhance the pepper root growth, increase the gene expressions of CaPR4 and CaPR10 after pathogen challenge for 6 h, but limited to suppress the pepper bacterial spot disease as compare to the mock-treated plants. Strain S2-3-2 can be a potential biological control agent on the plant root for plant growth promoting and disease suppression.

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Authors and Affiliations

  1. School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea

    Weilan Li, Seung-Yeol Lee & Hee-Young Jung

  2. Institute of Plant Medicine, Kyungpook National University, Daegu, 41566, Republic of Korea

    Seung-Yeol Lee & Hee-Young Jung

  3. School of Food Science and Biotechnology, Kyungpook National University, Daegu, 41566, Republic of Korea

    Young-Je Cho

  4. School of Life Sciences, Kyungpook National University, Daegu, 41566, Republic of Korea

    Sa-Youl Ghim

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  1. Weilan Li
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Correspondence to Hee-Young Jung.

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Li, W., Lee, SY., Cho, YJ. et al. Mediation of induced systemic resistance by the plant growth-promoting rhizobacteria Bacillus pumilus S2-3-2. Mol Biol Rep 47, 8429–8438 (2020). https://doi.org/10.1007/s11033-020-05883-9

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  • DOI: https://doi.org/10.1007/s11033-020-05883-9

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