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Development of a specific AFLP-based SCAR marker for Chinese Race 34MKG of Puccinia graminis f. sp. tritici

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Abstract

Wheat stem rust, caused by Puccinia graminis f. sp. tritici (Pgt), is a fungus that causes the devastating fungalwheat stem rust disease in wheat production. Rapid identification of the physiological races of Pgt are very importance for the prevention of wheat stem rust. In this paper we developed a molecular method to identify the most prevalent race of Pgt, as a supplement for traditionally used host-specific methods. Amplified fragment length polymorphism (AFLP) was employed as a means of analyzing DNA polymorphisms in six common physiological races of Pgt in China and Ug99. In total, 64 pairs of primers were used for AFLP screening of race-specific molecular markers. One primer pair-namely, E7/M7 (5′-GACTGCGTACCAATTCG G-3′/5′-GATGAGTCCTGAGTAACGG-3′)-yielded a unique band for the race 34MKG that was purified and cloned into the pGEM-T vector for sequencing. We then designed a new primer pairs (sequence—characterized amplified region marker) to amplify the 171-bp fragment and confirmed that the marker was highly specific for 34MKG. These results provide a new tool for monitoring different races of Pgt for improved control of wheat stem rust in China.

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Funding

This study was supported by the Heilongjiang Academy of Agricultural Sciences Doctoral Research start-up fund, the National Natural Science Foundation of China (31701738), the National Natural Science Foundation of China (31471546) and Harbin science and technology bureau planning project (2016AE6AE001).

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Authors and Affiliations

  1. Industrial Crops Institute of Heilongjiang Academy of Agricultural Sciences, Harbin, 150086, Heilongjiang, China

    Si Chen, Xue Yang, Wen-gong Huang, Yan Liu, Ying Yu, Guang-wen Wu & Shu-quan Zhang

  2. College of Plant Protection, Shenyang Agricultural University, Shenyang, 110866, Liaoning, China

    Tian-ya Li & Yuan-yin Cao

Authors
  1. Si Chen
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  2. Xue Yang
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  3. Wen-gong Huang
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  4. Yan Liu
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  5. Ying Yu
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  6. Guang-wen Wu
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  7. Shu-quan Zhang
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  8. Tian-ya Li
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  9. Yuan-yin Cao
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Correspondence to Tian-ya Li or Yuan-yin Cao.

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Chen, S., Yang, X., Huang, Wg. et al. Development of a specific AFLP-based SCAR marker for Chinese Race 34MKG of Puccinia graminis f. sp. tritici. Mol Biol Rep 47, 4303–4309 (2020). https://doi.org/10.1007/s11033-020-05513-4

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  • DOI: https://doi.org/10.1007/s11033-020-05513-4

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