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Microsatellite primer development in elasmobranchs using next generation sequencing of enriched libraries

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Abstract

Microsatellites are useful in studies of population genetics, sibship, and parentage. Here, we screened for microsatellites from multiple elasmobranch genomic libraries using an enrichment protocol followed by sequencing on an Illumina platform. We concurrently screened five and then nine genomes and describe the number of potential loci from each respective round of sequencing. To validate the efficacy of the protocol, we developed and tested primers for the pelagic thresher shark, Alopias pelagicus. The method described here is a cost-effective protocol to increase the pool of potential useful loci and allows the concurrent screening of multiple libraries.

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Acknowledgements

Genetic research was carried out in the Field Museum’s Pritzker Laboratory for Molecular Systematics and Evolution operated with support from the Pritzker Foundation. Data were collected on an Illumina MiSeq, generously donated to the Pritzker Lab by René and Bruce Lauer. We thank four anonymous reviewers for comments on previous versions of the manuscript.

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Authors and Affiliations

  1. Pritzker Laboratory for Molecular Systematics and Evolution, Field Museum of Natural History, 1400 South Lake Shore Dr., Chicago, IL, 60605, USA

    Kevin A. Feldheim & J. Dylan Maddox

  2. Elasmo Project, P.O. Box 29588, Dubai, United Arab Emirates

    Rima W. Jabado

  3. Department of Biological Sciences, Florida International University, North Miami, FL, 33181, USA

    Demian D. Chapman

  4. School of Marine and Atmospheric Sciences, Stony Brook University, Stony Brook, NY, 11794, USA

    Diego Cardeñosa

  5. Environmental Sciences, American Public University System, Charles Town, WV, 25414, USA

    J. Dylan Maddox

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  1. Kevin A. Feldheim
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Correspondence to Kevin A. Feldheim.

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Feldheim, K.A., Jabado, R.W., Chapman, D.D. et al. Microsatellite primer development in elasmobranchs using next generation sequencing of enriched libraries. Mol Biol Rep 47, 2669–2675 (2020). https://doi.org/10.1007/s11033-020-05357-y

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