Abstract
A consistent kojic acid producing fungal strain has been isolated from rice husk using glucose-peptone medium. The isolate was identified as Aspergillus sojae SSC-3 on 18S rDNA analysis. A. sojae was capable of producing substantially good amount of kojic acid, however the production was varying from batch to batch. In order to obtain consistent, repeated and high levels of kojic acid, monospore isolation procedures was adopted. The highest production of kojic acid obtained was 12 ± 2 g/L in 120 h with sucrose (10%) and yeast extract (0.5%) as carbon and nitrogen source respectively. The process was scale up to 10 L fermenter size which repeatedly resulted in the production of 18 ± 2 g/L of kojic acid in 96 h. Kojic acid was recovered (> 82%) from the fermentation broth with > 99% purity. Best to our knowledge this is the first report were kojic acid production is reported from Aspergillus sojae strain.
Similar content being viewed by others
References
Lee YS, Park JH, Kim MH, Seo SH, Kim HJ (2006) Synthesis of tyrosinase inhibitory kojic acid derivative. Arch Pharm 339(3):111–114
Noh JM, Kwak SY, Seo HS, Seo JH, Kim BG, Lee YS (2009) Kojic acid–amino acid conjugates as tyrosinase inhibitors. Bioorg Med Chem Lett 19(19):5586–5589
Radhakrishnan SK, Shimmon RG, Conn C, Baker AT (2016) Evaluation of novel chalcone oximes as inhibitors of tyrosinase and melanin formation in B16 cells. Arch Pharm 349(1):20–29
Mohamad R, Mohamed MS, Suhaili N, Salleh MM, Ariff AB (2010) Kojic acid: applications and development of fermentation process for production. Biotechnol Mol Biol Rev 5(2):24–37
Panich U, Onkoksoong T, Kongtaphan K, Kasetsinsombat K, Akarasereenont P, Wongkajornsilp A (2011) Inhibition of UVA-mediated melanogenesis by ascorbic acid through modulation of antioxidant defense and nitric oxide system. Arch Pharm Res 34(5):811–820
Mohamad R, Mohamed MS, Suhaili N, Salleh MM, Ariff AB (2010) Kojic acid: Applications and development of fermentation process for production. Biotechnol Mol Biol Rev 5(2):24–37
Rahimi VB, Askari VR, Emami SA, Tayarani-Najaran Z (2017) Anti-melanogenic activity of Viola odorata different extracts on B16F10 murine melanoma cells. Iran J Basic Med Sci 20(3):242
Gulsah K, Ayse E, Selin O, Dilsiz AM (2018) Synthesis and cytotoxic evaluation of kojic acid derivatives with inhibitory activity on melanogenesis in human melanoma cells. Anti-cancer Agents Med Chem 18:2137–21048
Boissy RE, Visscher M, DeLong MA (2005) DeoxyArbutin: a novel reversible tyrosinase inhibitor with effective in vivo skin lightening potency. Exp Dermatol 14(8):601–608
Khazaeli P, Goldoozian R, Sharififar F (2009) An evaluation of extracts of five traditional medicinal plants from Iran on the inhibition of mushroom tyrosinase activity and scavenging of free radicals. Int J Cosmet Sci 31(5):375–381
Hseu YC, Cheng KC, Lin YC, Chen CY, Chou HY, Ma DL et al (2015) Synergistic effects of linderanolide b combined with arbutin, ptu or kojic acid on tyrosinase inhibition. Curr Pharm Biotechnol 16(12):1120–1126
Chung KW, Jeong HO, Lee EK, Kim SJ, Chun P, Chung HY, Moon HR (2018) Evaluation of antimelanogenic activity and mechanism of galangin in silico and in vivo. Biol Pharm Bull 41(1):73–79
Wilson BJ (1971) Miscellaneous Aspergillus toxins. In: Kadis S, Ciegler A, Aji SJ (eds) Microbes toxins, fungal toxins. Academic Press, New York, pp 235–250
Blumenthal CZ (2004) Production of toxic metabolites in Aspergillus niger, Aspergillus oryzae, and Trichoderma reesei: justification of mycotoxin testing in food grade enzyme preparations derived from the three fungi. Regul Toxicol Pharmacol 39(2):214–228
Bentley R (2006) From miso, sake and shoyu to cosmetics: a century of science for kojic acid. Nat Prod Rep 23(6):1046–1062
Terabayashi Y, Sano M, Yamane N, Marui J, Tamano K, Sagara J, Higa Y (2010) Identification and characterization of genes responsible for biosynthesis of kojic acid, an industrially important compound from Aspergillus oryzae. Fungal Genet Biol 47(12):953–961
Yamada R, Yoshie T, Wakai S, Asai-Nakashima N, Okazaki F, Ogino C, Kondo A (2014) Aspergillus oryzae-based cell factory for direct kojic acid production from cellulose. Microb Cell Fact 13(1):71
Liu X, Jiang Q, Xia W (2018) One-step procedure for enhancing the antibacterial and antioxidant properties of a polysaccharide polymer: kojic acid grafted onto chitosan. Int J Biol Macromol 113:1125–1133
Kotani T, Ichimoto I, Tatsumi C, Fujita T (1976) Bacteriostatic activities and metal chelation of kojic acid analogs. Agric Biol Chem 40(4):765–770
Brtko J, Rondahl L, Fickova M, Hudecova D, Eybl V, Uher M (2004) Kojic acid and its derivatives: history and present state of art. Cent Eur J Public Health 12(SUPP):S16–S17
Reddy BS, Reddy MR, Madan CH, Kumar KP, Rao MS (2010) Indium (III) chloride catalyzed three-component coupling reaction: a novel synthesis of 2-substituted aryl (indolyl) kojic acid derivatives as potent antifungal and antibacterial agents. Bioorg Med Chem Lett 20(24):7507–7511
Lei JX, Wang J, Huo Y, You Z (2016) 4-Fluoro-N’-(2-hydroxy-3-methoxybenzylidene) benzohydrazide and its oxidovanadium (V) complex: syntheses, crystal structures and insulin-enhancing activity. Acta Chim Slov 63(3):670–677
Wei Y, Zhang C, Zhao P, Yang X, Wang K (2011) A new salicylic acid-derivatized kojic acid vanadyl complex: synthesis, characterization and anti-diabetic therapeutic potential. J Inorg Biochem 105(8):1081–1085
Sharma DK, Pandey J, Tamrakar AK, Mukherjee D (2014) Synthesis of heteroaryl/aryl kojic acid conjugates as stimulators of glucose uptake by GLUT4 translocation. Eur J Med Chem 85:727–736
Matsumoto M, Todo H, Akiyama T, Hirata-Koizumi M, Sugibayashi K, Ikarashi Y, Yokoyama K (2016) Risk assessment of skin lightening cosmetics containing hydroquinone. Regul Toxicol Pharmacol 81:128–135
Ariff AB, Webb C (1998) Effect of initial carbon and nitrogen sources concentrations on growth of Aspergillus awamori and glucoamylase production. Asia Pac J Mol Biol Biotechnol 6(2):161–169
El-Kady IA, Zohri ANA, Hamed SR (2014) Kojic acid production from agro-industrial by-products using fungi. Biotechnol Res Int. https://doi.org/10.1155/2014/642385
Morton HE, Kocholaty W, Junowicz-Kocholaty R, Kelner A (1945) Toxicity and antibiotic activity of kojic acid produced by Aspergillus luteo-virescens. J Bacteriol 50(5):579
Saleh RM, Kabli SA, Al-Garni SM, Mohamed SA (2011) Screening and production of antibacterial compound from Trichoderma spp against human-pathogenic bacteria. Afr J Microbiol Res 5(13):1619–1628
Ariff Arbakariya (2012) Purification of kojic acid. PI 2012000679
Spatafora JW, Robbertse B (2010) Phylogenetics and phylogenomics of the fungal tree of life. Cellular and Molecular Biology of Filamentous Fungi. American Society of Microbiology, Washington, DC, pp 36–49
Strom NB, Bushley KE (2016) Two genomes are better than one: history, genetics, and biotechnological applications of fungal heterokaryons. Fungal Biol Biotechnol 3(1):4
Kale SHUBHA, Bennett JW (1992) Strain instability in filamentous fungi. Handb Appl Mycol 5:311–332
Hazzaa MM, Saad AM, Hassan HM, Ibrahim E (2013) High Production of Kojic acid crystals by isolated Aspergillus oryzae var. effusus NRC14. J Appl Sci Res 9(3):17141723
Wei G, Kloepper JW, Tuzun S (1991) Induction of systemic resistance of cucumber to Colletotrichum orbiculare by select strains of plant growth-promoting rhizobacteria. Phytopathology 81(11):1508–1512
El-Aasar SA (2006) Cultural conditions studies on kojic acid production by Aspergillus parasiticus. Int J Agric Biol 8(4):468–473
Ariff AB, Salleh MS, Ghani B, Hassan MA, Rusul G, Karim MIA (1996) Aeration and yeast extract requirements for kojic acid production by Aspergillus flavus link. Enzym Microbial Technol 19(7):545–550
Rosfarizan M, Ariff AB, Hassan MA, Karim MIA (1998) Kojic acid production by Aspergillus flavus using gelatinized and hydrolyzed sago starch as carbon sources. Folia Microbiol 43(5):459–464
Ogawa A, Wakisaka Y, Tanaka T, Sakiyama T, Nakanishi K (1995) Production of kojic acid by membrane-surface liquid culture of Aspergillus oryzae NRRL484. J Ferment Bioeng 80(1):41–45
Acknowledgements
Authors thanks Director, CSIR-IIIM for his interest in this work and for providing the facilities to carry out the work. The authors SC and SS are thankful to SERB-DST-India EMR/2016/002786 for funding. IIIM Publication No IIIM/2295/2019.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
There is a not conflict of interest among authors for publication of this MS.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Chib, S., Dogra, A., Nandi, U. et al. Consistent production of kojic acid from Aspergillus sojae SSC-3 isolated from rice husk. Mol Biol Rep 46, 5995–6002 (2019). https://doi.org/10.1007/s11033-019-05035-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11033-019-05035-8