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Molecular cloning and oxidative-stress responses of a novel manganese superoxide dismutase (MnSOD) gene in the dinoflagellate Prorocentrum minimum

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Abstract

Dinoflagellate algae are microeukaryotes that have distinct genomes and gene regulation systems, making them an interesting model for studying protist evolution and genomics. In the present study, we discovered a novel manganese superoxide dismutase (PmMnSOD) gene from the marine dinoflagellate Prorocentrum minimum, examined its molecular characteristics, and evaluated its transcriptional responses to the oxidative stress-inducing contaminants, CuSO4 and NaOCl. Its cDNA was 1238 bp and contained a dinoflagellate spliced leader sequence, a 906 bp open reading frame (301 amino acids), and a poly (A) tail. The gene was coded on the nuclear genome with one 174 bp intron; signal peptide analysis showed that it might be localized to the mitochondria. Real-time PCR analysis revealed an increase in gene expression of MnSOD and SOD activity when P. minimum cells were separately exposed to CuSO4 and NaOCl. In addition, both contaminants considerably decreased chlorophyll autofluorescence, and increased intracellular reactive oxygen species. These results suggest that dinoflagellate MnSOD may be involved in protecting cells against oxidative damage.

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Fig. 1
Fig. 2

taken from GenBank database, and their accession numbers are provided in Supplementary Table 2. (Color figure online)

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Fig. 7

adapted from Sheshadri and Kumar (2016). CAT catalase, GPx glutathione peroxidase, GST glutathione S-transferase, GSSG glutathione disulphide

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Acknowledgements

We thank Dr. S. Abbasi for critical comments on the early version of manuscript. This work was supported by the National Research Foundation of Korea Grant funded by the Korean Government (2016R1D1A1A09920198), and by a grant from the National Institute of Fisheries Science (R2019037) funded to J.-S. Ki.

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  1. Department of Biotechnology, Sangmyung University, Seoul, 03016, South Korea

    Hui Wang, Hansol Kim & Jang-Seu Ki

  2. Ocean Climate and Ecology Research Division, National Institute of Fisheries Science (NIFS), Busan, 46083, South Korea

    Weol-Ae Lim

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Wang, H., Kim, H., Lim, WA. et al. Molecular cloning and oxidative-stress responses of a novel manganese superoxide dismutase (MnSOD) gene in the dinoflagellate Prorocentrum minimum. Mol Biol Rep 46, 5955–5966 (2019). https://doi.org/10.1007/s11033-019-05029-6

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