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Genome-wide identification and expression profiling of the late embryogenesis abundant genes in potato with emphasis on dehydrins

Molecular Biology Reports volume 42, pages 1163–1174 (2015)Cite this article

Abstract

Late embryogenesis abundant (LEA) proteins were first described as accumulating late in plant seed development. They were also shown to be involved in plant responses to environmental stress and as well as in bacteria, yeast and invertebrates. They are known to play crucial roles in dehydration tolerance. This study describes a genome-wide analysis of LEA proteins and the corresponding genes in Solanum tuberosum. Twenty-nine LEA family members encoding genes in the Solanum genome were identified. Phylogenetic analyses allowed the classification of the potato LEA proteins into nine distinct groups. Some of them were identified as putative orthologs of Arabidopsis and rice LEA genes. In silico analyses confirmed the hydrophilicity of most of the StLEA proteins, whereas some of them can be folded. The in silico expression analyses showed that the identified genes displayed tissue-specific, stress and hormone-responsive expression profiles. Five StLEA classified as dehydrins were selected for expression analyses under salt and drought stresses. The data revealed that they were induced by both stresses. The analyses indicate that several factors such us developmental stages, hormones, and dehydration, can regulate the expression and activities of LEA protein. This report can be helpful for the further functional diversity studies and analyses of LEA proteins in potato. These genes can be overexpressed to improve potato abiotic stress response.

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Acknowledgments

This work was financed by the Tunisian Ministry of High Education and Scientific Research. The authors are grateful to Anne-Lise Haenni from the Institute Jacques Monod (France) for reading and improving the English.

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Affiliations

  1. Unité Enzymes et Bioconversion, Ecole Nationale d’Ingénieurs de Sfax, Route Soukra Km 4, B.P 1173, 3038, Sfax, Tunisia

    Safa Charfeddine, Mariam Charfeddine & Radhia Gargouri-Bouzid

  2. Centre de Biotechnologie de Sfax, Route Sidi Mansour Km 4, B.P 1177, 3018, Sfax, Tunisia

    Mohammed Najib Saïdi

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  1. Safa Charfeddine
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  2. Mohammed Najib Saïdi
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  3. Mariam Charfeddine
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Correspondence to Safa Charfeddine.

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Charfeddine, S., Saïdi, M.N., Charfeddine, M. et al. Genome-wide identification and expression profiling of the late embryogenesis abundant genes in potato with emphasis on dehydrins. Mol Biol Rep 42, 1163–1174 (2015). https://doi.org/10.1007/s11033-015-3853-2

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  • DOI: https://doi.org/10.1007/s11033-015-3853-2

Keywords

  • Late embryogenesis abundant
  • Solanum tuberosum
  • Genome-wide analysis
  • Stress