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Biological characteristics of rat dorsal root ganglion cell and human vascular endothelial cell in mono- and co-culture

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Abstract

This study aimed to evaluate the biological activity of rat dorsal root ganglion cell (DRGC) and human vascular endothelial cell (HMVEC) in mono- and co-culture. Expression levels of vascular endothelial growth factor (VEGF) and nerve growth factor (NGF) mRNA were measured by quantitative real-time RT-PCR (qRT-PCR). Western blot analysis was used to identify VEGF and NGF protein expressions. Cell injury was assessed by measuring cell viability with methylthiazol tetrazolium (MTT) assay. The results showed that VEGF and NGF mRNA levels in the HMVEC+DRGC group were significantly higher than those in the DRGC and HMVEC groups (all p < 0.05). There were also greater increases in both VEGF and NGF protein expressions in the HMVEC+DRGC group than those in the DRGC and HMVEC groups (all p < 0.05). The results of MTT analysis revealed significant differences in cell viability among the HMVEC+DRGC group and the DRGC and HMVEC groups (all p < 0.05). In summary, our findings provide evidence that DRGC and HMVEC in co-culture may exhibit greater biological activity than DRGC in mono-culture.

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Acknowledgments

This work is suppprted by the Natural Science Foundation of Liaoning Province (201102252), the Social Development Research Plan of Liaoning Province (201225094), and the Program for Liaoning Excellent Talents in University, LNET. We would like to acknowledge the reviewers for their helpful comments on this paper.

Conflict of interests

The authors have declared that no competing interests exist.

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Authors and Affiliations

  1. Department of Orthopedics, Shengjing Hospital, China Medical University, San Hao Street No. 36, Heping District, Shenyang, 110004, People’s Republic of China

    Quan Yuan, Jian-Jun Li & Chun-Hou An

  2. Department of Nephrology, The First Affiliated Hospital of China Medical University, Shenyang, 110004, People’s Republic of China

    Li Sun

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  1. Quan Yuan
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  2. Jian-Jun Li
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Correspondence to Quan Yuan.

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Yuan, Q., Li, JJ., An, CH. et al. Biological characteristics of rat dorsal root ganglion cell and human vascular endothelial cell in mono- and co-culture. Mol Biol Rep 41, 6949–6956 (2014). https://doi.org/10.1007/s11033-014-3581-z

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  • DOI: https://doi.org/10.1007/s11033-014-3581-z

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