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TNF A −308G>A polymorphism in Moroccan patients with type 2 diabetes mellitus: a case–control study and meta-analysis

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Abstract

Type 2 diabetes mellitus (T2DM) is one of causes of mortality and morbidity in Moroccan population. The identification of genes implicated in this disease can help to found a specific treatment and to improve the quality of life for type 2 diabetic patients. In this study we analyze the association between a polymorphism (−308G>A) of TNF A promoter gene and T2DM in Moroccan patients. Five hundred and fifty-one individuals (307 patients with T2DM and 244 controls) were genotyped for this polymorphism by PCR–RFLP. This association was further reconsidered by a meta-analysis on 21 studies including 8,187 cases and 7,811 controls. We found that in Moroccan patients the −308A allele is strongly associated with T2DM (p = 0.000002; odds ratio 1.79, 95 % confidence interval 1.41–2.28). Based on our meta-analysis, there was no significant association detected between the TNF A −308G>A polymorphism and risk for T2DM. Our results suggest that the −308G>A polymorphism is a genetic risk factor for the development of T2DM in Moroccan population. On the other hand the meta analysis results led to controversial conclusions in other ethnicities.

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Acknowledgments

We acknowledge the family members involved in this study. This work was supported by Pasteur Institute of Morocco and a grant from the project NEPAD/NABNet (New Partnership for Africa’s Development, North Africa Biosciences Network).

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Laboratoire de Génétique Moléculaire Humaine, Département de Recherche Scientifique, Institut Pasteur du Maroc, 1, Place Louis Pasteur, 20360, Casablanca, Morocco

    Hajar Sefri, Houda Benrahma, Hicham Charoute, Fouzia Lakbakbi el yaagoubi, Hassan Rouba, Omar Abidi & Abdelhamid Barakat

  2. Laboratory of Physiology, Department of Pharmacology and Environmental Health, Faculty of Sciences, University Sidi Mohamed Ben Abdallah, Fez, Morocco

    Badiaa Lyoussi

  3. Medical Virology and BSL3 Laboratory, Institut Pasteur du Maroc, 1, Place Louis Pasteur, 20360, Casablanca, Morocco

    Jalal Nourlil

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  1. Hajar Sefri
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  2. Houda Benrahma
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Correspondence to Abdelhamid Barakat.

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Sefri, H., Benrahma, H., Charoute, H. et al. TNF A −308G>A polymorphism in Moroccan patients with type 2 diabetes mellitus: a case–control study and meta-analysis. Mol Biol Rep 41, 5805–5811 (2014). https://doi.org/10.1007/s11033-014-3454-5

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