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Molecular characterization, expression profiles, and analysis of Qinchuan cattle SIRT1 gene association with meat quality and body measurement traits (Bos taurus)

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Abstract

Silent information regulator (SIRT1), was closely associated with senescence, metabolism, and apoptosis. The objective of this study was to investigate whether SIRT1 gene could be used as a candidate gene in the breeding process of Qinchuan cattle. Via sequencing technology conducted in 453 individuals of Qinchuan cattle, single nucleotide polymorphisms (G25764A, A25846G, and T25868C) with 5 haplotypes and 6 combined genotypes in 3′ untranslated region of SIRT1 gene were identified. In addition, three loci were significantly associated with some of the body measurements and meat quality traits in Qinchuan cattle (P < 0.05), and the H2H2 (GG–AA–CC) diplotypes had better performance than other combinations in Qinchuan cattle. These results suggest that the SIRT1 gene could be used in marker assisted selection to improve the production traits of Qinchuan cattle.

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Acknowledgments

We are grateful to Professor Zan Linsen for providing the necessary materials used to accomplish this research work. The research was supported by the National “Five year” Science and Technology Support Project (#2011BAD28B04-03), the China National 863 Program (#2011AA100307), the GMO New Varieties Major Project (#2011ZX08007-002), the National Beef and Yak Industrial Technology System (#CARS-38), Chinese Beef Cattle Economically Important Traits Functional Genomics Studies Project (2013AA102505), Identification And Regulation of Qinchuan Cattle Meat Quality Traits Functional Gene Project (31272411).

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Authors and Affiliations

  1. College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, People’s Republic of China

    Linsheng Gui, Hongbao Wang, Shengjuan Wei, Yaran Zhang & Linsen Zan

  2. National Beef Cattle Improvement Center, Northwest A&F University, Yangling, Shaanxi, 712100, People’s Republic of China

    Linsen Zan

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  1. Linsheng Gui
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  2. Hongbao Wang
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  5. Linsen Zan
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Correspondence to Linsen Zan.

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Gui, L., Wang, H., Wei, S. et al. Molecular characterization, expression profiles, and analysis of Qinchuan cattle SIRT1 gene association with meat quality and body measurement traits (Bos taurus). Mol Biol Rep 41, 5237–5246 (2014). https://doi.org/10.1007/s11033-014-3393-1

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  • DOI: https://doi.org/10.1007/s11033-014-3393-1

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