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Different associations of apoE gene polymorphism with metabolic syndrome in the Vojvodina Province (Serbia)

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Abstract

The metabolic syndrome (MetS) is a polygenic multifactorial metabolic disorder with strong socioeconomic influence. MetS has became a worldwide epidemic, that directly increases the risk of cardiovascular diseases and type 2 diabetes mellitus. The human apoE gene, coding Apolipoprotein E, has three common polymorphisms in human population: e2, e3 and e4, which are proved to be associated with impaired lipid metabolism. The contribution of apoE polymorphism to MetS disorders has not been investigated previously in Vojvodina Province, region with the highest number of obese people in Serbia. The aim of this study was to evaluate apoE gene polymorphism in relation to MetS disorders. The healthy control group of 30 individuals and 63 MetS patients were examined for apoE variants in relation to biochemical and anthropometric parameters. The genotypes were determined by PCR–RFLP. Regarding all parameters, significantly higher values were detected in MetS group compared to control. The MetS group of patients had significantly higher frequency of e4 allele. In addition, positive relation was revealed between e4 allele presence and all measured parameters. It was found that the e4 allele was related with a significantly increased OR of MetS disorders according to the International Diabetes Federation definition. These results suggested that e4 allele may act as a one of determinants for development of metabolic syndrome.

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Acknowledgments

The completion of this study was made possible through the support of all the study participants and the research staff of the study. The study was supported by the Ministry of Education, Science and Technological Development, Republic of Serbia, Grant No. 43002 and The Provincial Secretariat for Science and Technological Development of Vojvodina, Grant No. 114-451-205672011. The authors are grateful to all of those who helped collecting the blood samples: Drita Skrbic nurse at the Department of Endocrinology, Diabetes and Metabolic Disorders, Novi Sad, Serbia.

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Authors and Affiliations

  1. Department of Pharmacy, Medical Faculty, University of Novi Sad, 21000, Novi Sad, Serbia

    Nataša Vučinić & Biljana Božin

  2. Institute of Oncology, 21104, Sremska Kamenica, Serbia

    Igor Djan

  3. Department of Endocrinology, Diabetes and Metabolic Disorders, Clinical Center of Vojvodina, Medical Faculty, University of Novi Sad, 21000, Novi Sad, Serbia

    Edita Stokić

  4. Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad, 21000, Novi Sad, Serbia

    Dragana Obreht & Mihajla Djan

  5. Clinical Center of Vojvodina, Medical Faculty, University of Novi Sad, Hajduk Veljkova 1, 21000, Novi Sad, Serbia

    Karmen Stankov

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  1. Nataša Vučinić
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  2. Igor Djan
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Correspondence to Nataša Vučinić.

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Vučinić, N., Djan, I., Stokić, E. et al. Different associations of apoE gene polymorphism with metabolic syndrome in the Vojvodina Province (Serbia). Mol Biol Rep 41, 5221–5227 (2014). https://doi.org/10.1007/s11033-014-3390-4

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  • DOI: https://doi.org/10.1007/s11033-014-3390-4

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