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Associations of polymorphisms in GHRL, GHSR, and IGF1R genes with feed efficiency in chickens

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Abstract

The ghrelin (GHRL), ghrelin receptor (GHSR), and insulin-like growth factor 1 receptor (IGF1R) genes have crucial effects on body weight (BW), body weight gain (BWG), feed intake (FI), and feed conversion ratio (FCR) in many species. However, few studies on associations of GHRL, GHSR, and IGF1R with BWG, FI, and FCR have been reported in chickens. In this study, 16 SNPs in GHRL, GHSR, and IGF1R genes were genotyped by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The objective of this study was to examine the associations of GHRL, GHSR, and IGF1R genes polymorphisms with BW at 49 days (BW49) and 70 days (BW70) of age, BWG, FI, and FCR in the interval in two yellow meat-type populations with a total of 724 birds. The results showed that rs15675067 of GHRL was significantly associated with BW70, BWG, and FCR (P < 0.05). For GHSR, rs16675844 had significant effects on FI and FCR (P < 0.01), and that rs14678932 showed significant association with BWG and FI (P < 0.05). Rs14011780 of IGF1R was strongly associated with BW49, BW70, and FCR (P < 0.05). Furthermore, haplotypes based on three SNPs of rs14986828, rs15675067, and rs15675065 in GHRL were significantly associated with BW70 and FCR (P < 0.05). Meanwhile, a three-SNP haplotype comprising rs14011783, rs14011780, and rs14011776 in IGF1R showed significant effects on BW49, BW70, and FCR (P < 0.05). Therefore, it was concluded that the identified SNPs and analyzed haplotypes in this study might be useful for broiler breeding programs.

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Acknowledgments

We sincerely thank Drs. Dexiang Zhang and Congliang Ji at Guangdong Wens Nanfang Poultry Breeding Co., Ltd., China for providing experimental chickens. This study was supported in part by grants from Changjiang Scholars and Innovative Research in University of China (IRT0945 and IRT1191), the Chinese Universities Scientific Fund (2012QJ096), and National Natural Science Foundation of China (31201794 and 31172203).

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Authors and Affiliations

  1. National Engineering Laboratory for Animal Breeding and MOA Key Laboratory of Animal Genetics and Breeding, Department of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China

    Sihua Jin, Sirui Chen, Yue Lu, Guiyun Xu & Ning Yang

  2. College of Life Science, Shanxi Agricultural University, Taigu, 030801, China

    Huifeng Li

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  1. Sihua Jin
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  2. Sirui Chen
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  3. Huifeng Li
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  4. Yue Lu
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  6. Ning Yang
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Correspondence to Ning Yang.

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Sihua Jin and Sirui Chen contributed equally to the work.

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Jin, S., Chen, S., Li, H. et al. Associations of polymorphisms in GHRL, GHSR, and IGF1R genes with feed efficiency in chickens. Mol Biol Rep 41, 3973–3979 (2014). https://doi.org/10.1007/s11033-014-3265-8

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  • DOI: https://doi.org/10.1007/s11033-014-3265-8

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