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Systematic investigation of interactions between papain and MPA-capped CdTe quantum dots

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Abstract

Fluorescent quantum dots (QDs) have been widely applied in biological and biomedical areas, but relatively little is known about the interaction of QDs with some natural enzymes. Herein, the interactions between 3-mercaptopropionic acid-capped CdTe QDs (MPA-QDs) and papain were systematically investigated by UV–Vis absorption spectra, fluorescence spectra and circular dichroism (CD) spectra under the physiological conditions. The fluorescence spectra results indicated that MPA-QDs quenched the fluorescence intensity of papain. The modified Stern–Volmer quenching constant K a at different temperatures and the corresponding thermodynamic parameters ΔH, ΔG and ΔS were also calculated. The binding of MPA-QDs and papain is a result of the formation of QDs-papain complex and the electrostatic interactions play a major role in stabilizing the complex. The CD technique was further used to analyze the conformational changes of papain induced by MPA-QDs and the results indicated that the biological activity of papain was affected by MPA-QDs dramatically.

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Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (Grant No. 21203035, 21261005), the National Science Fund for Distinguished Young Scholars of China (Grant No. 21225313), Scientific Research Fund of Guangxi Provincial Education Department (2013YB138) and Guangxi Natural Science Foundation (2013GXNSFCA019005, 2013GXNSFBA019029).

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Authors and Affiliations

  1. College of Chemistry and Life Science, Guangxi Teachers Education University, Nanning, 530001, People’s Republic of China

    Qi Xiao, Hangna Qiu, Shan Huang, Chusheng Huang & Wei Su

  2. State Key Laboratory of Virology, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, People’s Republic of China

    Qi Xiao, Shan Huang & Yi Liu

  3. Key Laboratory of Beibu Gulf Environment Change and Resources Utilization (Guangxi Teachers Education University) Ministry of Education, Nanning, China

    Baoqing Hu

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  1. Qi Xiao
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Correspondence to Shan Huang or Yi Liu.

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Xiao, Q., Qiu, H., Huang, S. et al. Systematic investigation of interactions between papain and MPA-capped CdTe quantum dots. Mol Biol Rep 40, 5781–5789 (2013). https://doi.org/10.1007/s11033-013-2681-5

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  • DOI: https://doi.org/10.1007/s11033-013-2681-5

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