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Effect of Danshen aqueous extract on serum hs-CRP, IL-8, IL-10, TNF-α levels, and IL-10 mRNA, TNF-α mRNA expression levels, cerebral TGF-β1 positive expression level and its neuroprotective mechanisms in CIR rats

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Abstract

To observe the effects of Danshen aqueous extract (DSAE) on the cerebral tissue and nerve stem cells in cerebral ischemia reperfusion (CIR) rats. The model rats were prepared by occlusion of the middle cerebral artery for 2 h and then by reperfusion. They were randomly divided into five groups: a control group, an CIR group and three DSAE-treated groups. As compared with the sham control group, there was significant increase (P < 0.05, P < 0.01) in the serum high-sensitivity C-reactive protein (hs-CRP) and interleukin-8 (IL-8) levels, interleukin-10 (IL-10), tumor necrosis factor-alpha (TNF-α) levels, and IL-10 mRNA, TNF-α mRNA expression levels, function score, Infarct size, TUNEL + cell counts, cerebral transforming growth factor beta 1 (TGF-β1) positive expression and cerebral neuron specific enolase (NSE) levels, and decrease in fas-associated protein with death domain (FADD) and death-associated protein (Daxx) positive expression levels in the CIR group. Compared with CIR group, DSAE treatment dose-dependently significantly decreased serum hs-CRP, IL-8, IL-10, TNF-α levels, and IL-10 mRNA, TNF-α mRNA expression levels, function score, Infarct size, TUNEL + cell counts, cerebral TGF-β1 positive expression and cerebral NSE levels, and increase FADD and Daxx positive expression levels in the CIR + DSAE groups. Taken together, these results suggest that DSAE has a neuroprotective role in the CIR rats, which may be related to improvement of immunity function, proteins and genes expression.

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Authors and Affiliations

  1. Department of Neurology, General Hospital of Ningxia Medical University, Yinchuan, 750004, China

    Hai-Ning Li

  2. Department of Laboratory Medicine, General Hospital of Ningxia Medical University, Yinchuan, 750004, China

    Xue-Yun Liang & Xiao-Yan Yang

  3. Department of ICU, General Hospital of Ningxia Medical University, Yinchuan, 750004, China

    Wen-Yan Zhou

  4. Department of Anatomy Histology and Embryology, School of Basic Medical Science, Ningxia Medical University, Yinchuan, 750004, China

    Jian-Guo Niu

  5. Department of Hepatobiliary Surgery, General Hospital of Ningxia Medical University, Yinchuan, 750004, China

    Ben-Dong Chen

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Correspondence to Jian-Guo Niu.

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X.-Y. Liang, H.-N. Li and X.-Y. Yang equally contribute to this work.

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Liang, XY., Li, HN., Yang, XY. et al. Effect of Danshen aqueous extract on serum hs-CRP, IL-8, IL-10, TNF-α levels, and IL-10 mRNA, TNF-α mRNA expression levels, cerebral TGF-β1 positive expression level and its neuroprotective mechanisms in CIR rats. Mol Biol Rep 40, 3419–3427 (2013). https://doi.org/10.1007/s11033-012-2419-9

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