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Molecular and analysis of a phenylalanine ammonia-lyase gene (LrPAL2) from Lycoris radiata

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Abstract

Phenylalanine ammonia-lyase (PAL), the first enzyme of phenylpropanoid biosynthesis, participates in the biosynthesis of flavonoids, lignins, stilbenes and many other compounds. In this study, we cloned a 2,326 bp full-length PAL2 gene from Lycoris radiata by using degenerate oligonucleotide primer PCR (DOP-PCR) and the rapid amplification of cDNA ends method. The cDNA contains a 2,124 bp coding region encoding 707 amino acids. The LrPAL2 shares about 77.0 % nucleic acid identity and 83 % amino acid identity with LrPAL1. Furthermore, genome sequence analysis demonstrated that LrPAL2 gene contains one intron and two exons. The 5′ flanking sequence of LrPAL2 was also cloned by self-formed adaptor PCR (SEFA-PCR), and a group of putative cis-acting elements such as TATA box, CAAT box, G box, TC-rich repeats, CGTCA motif and TCA-element were identified. The LrPAL2 was detected in all tissues examined, with high abundance in bulbs at leaf sprouting stage and in petals at blooming stage. Besides, LrPAL2 drastically responded to MJ, SNP and UV, moderately responded to GA and SA, and a little increased under wounding. Comparison of LrPAL2 expression and LrPAL1 expression demonstrated that LrPAL2 can be more significantly induced than LrPAL1 under the above treatments, and LrPAL2 transcripts accumulated prominently at blooming stage, especially in petals, while LrPAL1 transcripts did not accumulated significantly at blooming stage. All these results suggested that LrPAL2 might play distinct roles in different branches of the phenylpropanoid pathway.

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Acknowledgments

This work was supported by China National Natural Science Foundation (Grant No. 31270339), by the National “863” program (Grant No. 2011AA10A206), and by Jiangsu Agricultural Science and Technology Independent Innovation Fund [No.CX (11)1016]. We are very grateful for their financial support.

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  1. Jiangsu Province Key Laboratory for Plant Ex-situ Conservation, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, 210014, China

    Yumei Jiang, Bing Xia, Lijian Liang, Xiaodan Li, Sheng Xu, Feng Peng & Ren Wang

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  1. Yumei Jiang
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  2. Bing Xia
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  3. Lijian Liang
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  4. Xiaodan Li
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  6. Feng Peng
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  7. Ren Wang
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Correspondence to Ren Wang.

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Jiang, Y., Xia, B., Liang, L. et al. Molecular and analysis of a phenylalanine ammonia-lyase gene (LrPAL2) from Lycoris radiata . Mol Biol Rep 40, 2293–2300 (2013). https://doi.org/10.1007/s11033-012-2310-8

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