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Transcriptome profiling of genes induced by salicylic acid and methyl jasmonate in Polygonum minus

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Abstract

The importance of plant secondary metabolites for both mankind and the plant itself has long been established. However, despite extensive research on plant secondary metabolites, plant secondary metabolism and its regulation still remained poorly characterized. In this present study, cDNA-amplified fragment length polymorphism (cDNA-AFLP) transcript profiling was applied to generate the expression profiles of Polygonum minus in response to salicylic acid (SA) and methyl jasmonate (MeJA) elicitations. This study reveals two different sets of genes induced by SA and MeJA, respectively where stress-related genes were proved to lead to the expression of genes involved in plant secondary metabolite biosynthetic pathways. A total of 98 transcript-derived fragments (TDFs) were up-regulated, including 46 from SA-treated and 52 from MeJA-treated samples. The cDNA-AFLP transcripts generated using 64 different Mse1/Taq1 primer combinations showed that treatments with SA and MeJA induced genes mostly involved in scavenging reactive oxygen species, including zeaxanthin epoxidase, cytosolic ascorbate peroxidase 1 and peroxidase. Of these stress-related genes, 15 % of other annotated TDFs are involved mainly in secondary metabolic processes where among these, two genes encoding (+)-delta cadinene synthase and cinnamoyl-CoA reductase were highlighted.

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Acknowledgments

This work was funded by Universiti Kebangsaan Malaysia through University Research Grants (UKM-AP-BPB-14-2009 and UKM-GUP-KPB-08-33-135) awarded to Prof. Dr. Normah Mohd Noor and Dr. Zamri Zainal respectively. Miss Su-Fang Ee is a recipient of the National Science Fellowship (NSF) granted by Ministry of Science, Technology and Innovation in Malaysia. Mr. Ji-Min Oh participated in the student exchange program supported by Rural Development Administration (RDA), Suwon, Korea. We thank Dr. Grantley W. Lycett from University of Nottingham, United Kingdom for proof reading the article.

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Authors and Affiliations

  1. School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600, UKM Bangi, Selangor, Malaysia

    Su-Fang Ee, Normah Mohd Noor, Zeti-Azura Mohamed-Hussein, Ismanizan Ismail & Zamri Zainal

  2. Institute of Systems Biology (INBIOSIS), Universiti Kebangsaan Malaysia, 43600, UKM Bangi, Selangor, Malaysia

    Su-Fang Ee, Normah Mohd Noor, Zeti-Azura Mohamed-Hussein, Ismanizan Ismail & Zamri Zainal

  3. National Institute of Crop Science, Rural Development Administration (RDA), Suwon, 441-100, Korea

    Ji-Min Oh & Taek-Ryoun Kwon

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  1. Su-Fang Ee
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Correspondence to Su-Fang Ee or Zamri Zainal.

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Ee, SF., Oh, JM., Mohd Noor, N. et al. Transcriptome profiling of genes induced by salicylic acid and methyl jasmonate in Polygonum minus . Mol Biol Rep 40, 2231–2241 (2013). https://doi.org/10.1007/s11033-012-2286-4

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  • DOI: https://doi.org/10.1007/s11033-012-2286-4

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