Abstract
Current study was conducted to investigation of effect of magnetic field on cell dedifferentiation and follow it callus induction derived from mature embryo culture in bread wheat genotypes. For this purpose, a factorial experiment based on completely randomized design was carried out with two wheat genotypes and three level of magnetic field strength (0.0, 8.8 and 17.6 Tesla) in three replications. Callus growth rate (CGR), relative growth rate (RGR), callus relative growth rate (CRGR), percentage of callus water content and percentage of callus induction traits were measured. To sum up, the results showed that differences between wheat genotypes and level of magnetic field strength were significant for some studied treats related to callus induction. The effect of magnetic field levels on CGR (from 0.181 to 0.175), RGR (from 1.442 to 0.655) and CRGR (from 0.052 to 0.022) were decreased with increment of magnetic field intensity.
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Abbreviations
- CGR:
-
Callus growth rate
- RGR:
-
Relative growth rate
- CRGR:
-
Callus relative growth rate
- PCWC:
-
Percentage of callus water content
- POCI:
-
Percentage of callus induction
- S.O.V:
-
Source of variations
- d.f.:
-
Degree of freedom
- CV:
-
Coefficient of variation
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Acknowledgments
The authors would like for gratefully acknowledge Mr Gholamreza Amjadian for his helpful comments and preparing the magnetic coil. Razi University has financially supported this research project No. 868. Thanks to The Cooperative No. 4407 the Knowledge Base New Ideas Co. and The Cooperative the Knowledge Base Shafa Hooraman for all supports.
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Kahrizi, D., Cheghamirza, K., Akbari, L. et al. Effects of magnetic field on cell dedifferentiation and callus induction derived from embryo culture in bread wheat (Triticum aestivum. L) genotypes. Mol Biol Rep 40, 1651–1654 (2013). https://doi.org/10.1007/s11033-012-2214-7
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DOI: https://doi.org/10.1007/s11033-012-2214-7