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Effect of PI3K gene silencing on growth, migration and related proteins expression of CD40 signal-mediated gastric cancer cells

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Abstract

In this study, we investigate effect of PI3K gene silencing on growth, migration and related proteins expression of CD40 signal-mediated gastric cancer cells. We observed that combination of sCD40L with PI3K siRNA could significantly inhibit AGS cells growth, block cells in G1 phase, and promote tumour cells apoptosis after 24 h treatment. Transwell test showed that numbers of cells per visual field in group PI3K siRNA or group sCD40L (after 24 h PI3K siRNA or sCD40L alone treatment) were fewer than that (32.54 ± 4.22) in control group. Numbers of cells per visual field in (after 24 h combination treatment of PI3K siRNA with sCD40L) were significantly fewer than that in group PI3K siRNA or group sCD40L. Compared with group sCD40L, expression level of Fas protein in group sCD40L + PI3K siRNA was significantly increased. The findings suggest that PI3K siRNA may strengthen CD40-induced specific antitumour effect via blocking PI3K/Akt signal pathway, resisting tumour immunoediting regulated by CD40 signal. Combination of sCD40L and PI3K siRNA is an important mechanism of gastric cancer therapy.

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Acknowledgments

The work was supported by the National Natural Science Foundation of China (No. 31270919) and the Natural Science Foundation of Jiangsu Province (BK2010231).

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Authors and Affiliations

  1. Department of Gastroenterology, The First Affiliated Hospital of Soochow University, Key Laboratory of Medicine and Clinical Immunology of Jiangsu Province, Suzhou, 215006, China

    Rui Li, Wei-Chang Chen, Xue-Qin Pang & Wen-Yan Tian

  2. College of Pharmacy, Soochow University, Suzhou, 215007, China

    Wei-Peng Wang

  3. Key Laboratory of Medicine and Clinical Immunology of Jiangsu Province, Institute of Medical Biotechnology, Soochow University, Suzhou, 215007, China

    Xue Guang Zhang

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  1. Rui Li
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Correspondence to Wei-Chang Chen.

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Li, R., Chen, WC., Pang, XQ. et al. Effect of PI3K gene silencing on growth, migration and related proteins expression of CD40 signal-mediated gastric cancer cells. Mol Biol Rep 40, 999–1008 (2013). https://doi.org/10.1007/s11033-012-2141-7

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  • DOI: https://doi.org/10.1007/s11033-012-2141-7

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