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Effects of retrograde gene transfer of brain-derived neurotrophic factor in the rostral spinal cord of a compression model in rat

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Abstract

Recovery after spinal cord injury (SCI) is rare in humans and experimental animals. Following SCI in adults, changes in gene expression and the regulation of these genes are associated with the pathological development of the injury. High levels of brain-derived neurotrophic factor (BDNF) in the injury area during the post-injury period contribute to enhanced neuroprotection and axonal regeneration. Intervention at the level of gene regulation has the potential to promote SCI repair. In this study, the injection of adenovirus-mediated BDNF in the lesion area (rostral spinal cord) up-regulated the expression of BDNF in the injury zone of a compression model in rat, thereby protecting neurons and enhancing behavioral function.

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Acknowledgments

We gratefully acknowledge Dr. Weiping Chen and Dr. Xiang Zhao for their assistance in animal experiments and in the scoring. Project supported by the National Natural Science Foundation of China (Grant No. 30901531) and the Natural Science Foundation of Zhejiang, China (Grant No. Y207216).

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Authors and Affiliations

  1. The Second Affiliated Hospital (Binjiang Branch), Hangzhou Binjiang Hospital, Zhejiang University School of Medicine, Hangzhou, 310000, China

    Tengfei Zhao & Xuesong Dai

  2. Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310009, China

    Yan Li, Xuesong Dai, Yiying Qi, Jianwei Wang & Kan Xu

  3. Department of Clinical Medicine, School of Medicine and Life Sciences, Zhejiang University City College, Hangzhou, 310015, China

    Junbo Wang

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  1. Tengfei Zhao
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  2. Yan Li
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  3. Xuesong Dai
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  4. Junbo Wang
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  5. Yiying Qi
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  6. Jianwei Wang
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  7. Kan Xu
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Corresponding authors

Correspondence to Xuesong Dai or Kan Xu.

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Zhao, T., Li, Y., Dai, X. et al. Effects of retrograde gene transfer of brain-derived neurotrophic factor in the rostral spinal cord of a compression model in rat. Mol Biol Rep 39, 8045–8051 (2012). https://doi.org/10.1007/s11033-012-1651-7

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  • DOI: https://doi.org/10.1007/s11033-012-1651-7

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