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Three Brassica rapa metallothionein genes are differentially regulated under various stress conditions

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Abstract

The expression profiles of three Brassica rapa metallothionein genes (BrMT 1–3) were determined in 7-day-old seedlings exposed to various exogenous factors including plant hormones, heavy metals and abiotic stresses. BrMT1, BrMT2, and BrMT3 were representatives of MT gene type 1, type 2, and type 3, respectively, according to their cysteine alignment. BrMT2 showed a relatively higher basal expression level compared to BrMT1 and BrMT3 under normal conditions. The BrMT1 transcript was markedly increased by various factors including ethephon, polyethylene glycol and hydrogen peroxide, with no down-regulation evident. On the contrary, BrMT2 expression was down-regulated by abscisic acid, salicylic acid, and methyl jasmonate. Heavy metals did not increase BrMT2 expression. BrMT3 expression was only marginally and non-significantly up- and down-regulated by the stress conditions tested. Promoter regions of BrMT1 and BrMT2 display different cis-acting elements supporting the different responses of both genes against various stresses. The results demonstrate the differential regulation of BrMT1–3 by various plant exogenous factors, and indicate the utility of the BrMT1 promoter as a multiple stress inducible promoter.

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Abbreviations

Br:

Brassica rapa

MT:

Metallothionein

PC:

Phytochelatins

PEG:

Polyethylene glycol

ROS:

Reactive oxygen species

SA:

Salicylic acid

ABA:

Abscisic acid

MeJA:

Methyl jasmonate

ET:

Ethephon

H2O2 :

Hydrogen peroxide

UBQ:

Ubiquitin

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Acknowledgments

This work was supported by the KRIBB initiative program and grant from Cabbage Genomics Assisted Breeding Supporting Research Center funded to H. Kim by Ministry for Food, Agriculture, Forestry and Fisheries, Korea.

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Authors and Affiliations

  1. Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Gwahangno 125, Daejeon, 305-806, Korea

    Young Ock Ahn, Sun Ha Kim, Haeng-Soon Lee & Sang-Soo Kwak

  2. Plant Systems Engineering Research Center and Cabbage Genomics Assisted Breeding Supporting Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Gwahangno 125, Daejeon, 305-806, Korea

    Jeongyeo Lee & HyeRan Kim

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  1. Young Ock Ahn
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Correspondence to Sang-Soo Kwak.

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Ahn, Y.O., Kim, S.H., Lee, J. et al. Three Brassica rapa metallothionein genes are differentially regulated under various stress conditions. Mol Biol Rep 39, 2059–2067 (2012). https://doi.org/10.1007/s11033-011-0953-5

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  • DOI: https://doi.org/10.1007/s11033-011-0953-5

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