Abstract
B-cell translocation gene 2 (BTG2), a member of the B-cell translocation gene family with anti-proliferative properties, have been characterized to be involved in cell growth, differentiation and survival. In this study, we cloned the full length sequences of cDNA and genomic DNA of BTG2 gene from the porcine skeletal muscle. Spatial expression analysis showed that the porcine BTG2 gene is expressed predominantly in muscle. Temporal expression analysis in longissimus dorsi muscle demonstrated that the expression of BTG2 gene has the highest expression at 60 days old in Large White while with a peak expression at 120 days old in Meishan. Temporal analysis also revealed that the expression of BTG2 gene is generally higher in Large White than in Meishan at all the developmental stages tested (65 days of conception and 3, 35, 60, 120, and 180 days of postnatal). A single nucleotide polymorphism (G417C) in the intron of BTG2 gene was then detected by PCR-RFLP in Large White × Meishan F2 resource population and association analysis suggested that this polymorphic site had significant association (P < 0.05) with the buttock fat thickness, fat percentage, lean muscle percentage, ratio of lean to fat and carcass length.
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Acknowledgments
We thank the Monitoring and Testing Center of Breeding Pig Quality of Wuhan, China providing the phenotypic data of F2 pigs. This work was supported financially by the National High Technology Research and Development Program of China Grant (2006AA10Z1D6), National Natural Science Foundation of China (30400313) and the creative team project of Education Ministry of China (IRT0831).
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X. Y. Mo and J. Lan contributed equally to this work.
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Mo, X.Y., Lan, J., Jiao, Q.Z. et al. Molecular characterization, expression pattern and association analysis of the porcine BTG2 gene. Mol Biol Rep 38, 4389–4396 (2011). https://doi.org/10.1007/s11033-010-0566-4
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DOI: https://doi.org/10.1007/s11033-010-0566-4