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Molecular characterization and NF-κB-regulated transcription of selenoprotein S from the Bama mini-pig

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Abstract

Selenoprotein S (SelS), a member of selenoprotein family, plays important regulatory function in inflammation and metabolic diseases. SelS expression is up-regulated response to the inflammatory stimulus in many mammal cells, animal models as well as patients. In order to further understand the function of SelS gene, molecular characterization and transcriptional regulation of SelS from a Bama mini-pig were analyzed in the present study. The results showed that pig SelS encoded a protein of 190 amino acid with estimated molecular weight of 21.23 kDa and pI of 9.526. The genomic structure, promoter and deduced amino acid sequence were analyzed and found to share high similarity with those of human SelS. Pig SelS fusion protein was demonstrated to localize in the cytoplasm by fluorescence microscopy. Real-time PCR revealed the ubiquitous expression pattern of pig SelS in diverse tissues, a high level expression was observed in the liver and lung, relatively low expression in other tissues, especially in muscle. Promoter deletion analysis further suggests that an NF-κB binding site within the SelS promoter is responsible for the up-regulation of SelS transcription.

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Acknowledgments

This work was supported by The National Natural Science Foundation of China (30800779, 30628019), The Ministry of Science and Technology of China (2008AA10Z143, 2009CB941604) and The Funding of State Key Laboratory of Animal Nutrition of China.

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Authors and Affiliations

  1. The State Key Laboratory for Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, People’s Republic of China

    Ningbo Zhang, Wenqian Jing, Jiayue Cheng, Wentao Cui, Yulian Mu & Kui Li

  2. College of Life Science, Linyi Normal University, Linyi, 276005, Shandong, People’s Republic of China

    Wenqian Jing

  3. College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Jiayue Cheng

  4. International Research Center of Future Agriculture for Human Health, Sichuan Agricultural University, Chengdu, 611130, People’s Republic of China

    Xingen Lei

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  1. Ningbo Zhang
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Correspondence to Kui Li.

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Zhang, N., Jing, W., Cheng, J. et al. Molecular characterization and NF-κB-regulated transcription of selenoprotein S from the Bama mini-pig. Mol Biol Rep 38, 4281–4286 (2011). https://doi.org/10.1007/s11033-010-0551-y

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  • DOI: https://doi.org/10.1007/s11033-010-0551-y

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