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Isolation and characterization of a novel BcMF14 gene from Brassica campestris ssp. chinensis

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Abstract

A putative RALF (rapid alkalinization factor)-like gene (GenBank accession number EF523517), named BcMF14, was isolated from Chinese cabbage (Brassica campestris L. ssp. chinensis Makino, syn. B. rapa ssp. chinensis) by rapid amplification of cDNA ends (RACE) based on a cDNA-AFLP differential fragment exclusively expressed in fertile line. Semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) discovered that BcMF14 was prominently expressed in stage four and five flower buds of fertile line, no expression in vegetative structures or in sterility line. Detailed RT-PCR illuminated its strong expression in stamens. Successful suppression of BcMF14 gene expression greatly reduced the normal pollen grains. The frequency of abnormal pollen grains was 48.95% in the mutant with many shriveled pollen grains with irregular shape and some larger ones with deep hollows along the germination ditch. Pollen germination was stopped because of the severely twisted pollen tubes. These results demonstrate a potential role of the BcMF14 gene in the development of male gametogenesis in Chinese cabbage.

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Acknowledgments

This research was supported by the Natural Science Foundation of China (No. 31000132) and the Key Sci-technology Project of Zhejiang Province, China (No. 2005C12019-02).

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Authors and Affiliations

  1. School of Life Sciences, Fuyang Teachers College, Fuyang, 230032, China

    Yanyan Li & Chuanpeng Nie

  2. Laboratory of Cell and Molecular Biology, Institute of Vegetable Science, Zhejiang University, Hangzhou, 310029, China

    Yanyan Li & Jiashu Cao

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  1. Yanyan Li
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  2. Chuanpeng Nie
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Correspondence to Yanyan Li.

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Li, Y., Nie, C. & Cao, J. Isolation and characterization of a novel BcMF14 gene from Brassica campestris ssp. chinensis . Mol Biol Rep 38, 1821–1829 (2011). https://doi.org/10.1007/s11033-010-0298-5

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