Abstract
Genetic association analysis was applied to examine the effect of the Na+/K+-ATPase beta 2 subunit (ATP1B2) gene on rectal temperature, milk traits, K+ levels and Na+/K+-ATPase (NKA) activity in the red blood cells of 1001 Chinese Holstein cows under normal and heat-stress conditions. We detected two novel single nucleotide polymorphisms, G2258A and C2833T, in the second and fourth introns, respectively, of ATP1B2. G2258A significantly affected milk fat content (P < 0.05) and 305-day milk yield (P < 0.01), but not milk protein content. C2833T significantly affected milk protein content (P < 0.01) and 305-day milk yield (P < 0.05), but not milk fat content. Calculated gene substitution effects suggested that A to G substitution in G2258A, and T to C substitution in C2833T, positively affected milk fat content, 305-day milk yield and somatic cell score, but negatively affected milk protein content. We also detected significant variation in milk fat content, milk protein content, 305-day milk yield and somatic cell scores (P < 0.05 or P < 0.01) among the nine ATP1B2 haplotypes. Under heat-stress, the C2833T polymorphism was significantly related to rectal temperature (P < 0.01), red blood cell K+ levels, NKA activity and milk yield (P < 0.05). Cows with the TT genotype showed the desirable characteristics of low rectal temperature and red blood cell K+, low decline rate in milk yield and red blood cell NKA activity. This study suggests that the ATP1B2 single nucleotide polymorphism C2833T is a genetic marker of heat-resistance traits in Chinese Holstein cows.
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Acknowledgments
This research was supported by the Science and Technology Sustentation Project of China (2006BAD04A01, 2006BAD04A12), the Science and Technology Sustentation Project of Jiangsu (BE2008306-2), the 863 High Technology and Development Project of China (2007AA10Z169) and the High Technology Independent Innovation Project from Shandong Academy of Agricultural Sciences (No. 2007YCX019).
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Wang, Z., Wang, G., Huang, J. et al. Novel SNPs in the ATP1B2 gene and their associations with milk yield, milk composition and heat-resistance traits in Chinese Holstein cows. Mol Biol Rep 38, 1749–1755 (2011). https://doi.org/10.1007/s11033-010-0289-6
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DOI: https://doi.org/10.1007/s11033-010-0289-6