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Molecular analysis of two salt-responsive NAC-family genes and their expression analysis in tomato

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Abstract

The NAC family transcription factor has demonstrated its importance in plant development and environmental stress response. Based on the microarray results under salt stress and EST information, the full-length cDNAs of two salt-inducible NAC-family genes (SlNAC1, SlNAM1) were isolated from a salt tolerant tomato cultivar, Edkawi, using Rapid Amplification of cDNA Ends (RACE). SlNAC1 and SlNAM1 encoded 301 and 296 amino acids, respectively, and the deduced protein sequences contained the typical domain of NAC-family transcription factors. Tissue expression profile analysis using semi-quantitative RT-PCR showed that SlNAC1 was expressed mainly in root, flower and green fruit; transcripts of SlNAM1 were detected in all tested tissues except for root, and high-level expression was detected in flower and matured tomato fruit. Both SlNAC1 and SlNAM1 were induced by salt stress in Edkawi, while the expression pattern was different in a salt-sensitive cultivar, ZS-5. Phylogenetic analysis for putative NAC-family peptides available in the tomato genome indicated a wide diversity of this gene family. Results obtained in the present study suggest that both SlNAC1 and SlNAM1 might play important roles in tomato stress tolerance.

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Acknowledgments

This work was supported by the Natural Science Foundation of China (No. 30400299 and No. 30771461).

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Authors and Affiliations

  1. College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, 430070, China

    Rongchao Yang, Chunting Deng, Bo Ouyang & Zhibiao Ye

  2. National Center for Vegetable Improvement (Central China), Huazhong Agricultural University, Wuhan, 430070, China

    Rongchao Yang, Chunting Deng, Bo Ouyang & Zhibiao Ye

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  1. Rongchao Yang
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  2. Chunting Deng
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  3. Bo Ouyang
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  4. Zhibiao Ye
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Correspondence to Bo Ouyang.

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11033_2010_177_MOESM1_ESM.jpg

Gene structure of SlNAC1 (a) and SlNAM1 (b). Exons are represented as black boxes. Numbers in boxes indicate exon size in base pairs (bp). Introns are shown as lines linking exons and their sizes are shown in base pairs. The start codon is shown as ATG, and stop codon is TAA for SlNAC1 and TGA for SlNAM1. NAC or NAM domain is shown as line or dot line below the exons, and numbers on the left indicate amino acid positions of the protein domains (JPG 550 kb)

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Yang, R., Deng, C., Ouyang, B. et al. Molecular analysis of two salt-responsive NAC-family genes and their expression analysis in tomato. Mol Biol Rep 38, 857–863 (2011). https://doi.org/10.1007/s11033-010-0177-0

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  • DOI: https://doi.org/10.1007/s11033-010-0177-0

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