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Cloning and expression analysis of phytoene desaturase and ζ-carotene desaturase genes in Carica papaya

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An Erratum to this article was published on 24 July 2012

Abstract

The fruit flesh color of papaya is an important nutritional quality trait and is due to the accumulation of carotenoid. To elucidate the carotenoid biosynthesis pathway in Carica papaya, the phytoene desaturase (PDS) and the ζ-carotene desaturase (ZDS) genes were isolated from papaya (named CpPDS and CpZDS) using the rapid amplification of cDNA ends (RACE) approach, and their expression levels were investigated in red- and yellow-fleshed papaya varieties. CpPDS contains a 1749 bp open reading frame coding for 583 amino acids, while CpZDS contains a 1716 bp open reading frame coding for 572 amino acids. The deduced CpPDS and CpZDS proteins contain a conserved dinucleotide-binding site at the N-terminus and a carotenoid-binding domain at the C-terminus. Papaya genome sequence analysis revealed that CpPDS and CpZDS are single copy; the CpPDS was mapped to papaya chromosome LG6, and the CpZDS was mapped to chromosome LG3. Quantitative PCR showed that both CpPDS and CpZDS were expressed in all tissues examined with the highest expression in maturing fruits, and that the expression of CpPDS and CpZDS were higher in red-fleshed fruits than in yellow-fleshed fruits. These results indicated that the differential accumulation of carotenoids in red- and yellow-fleshed papaya varieties might be partly explained by the transcriptional level of CpPDS and CpZDS.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (30760134, 30960218) and the Hainan Province Natural Science Foundation (808186).

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Authors and Affiliations

  1. Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Science, Haikou, 571101, China

    P. Yan, X. Z. Gao, W. T. Shen & P. Zhou

  2. Department of Basic Medical Science, Hainan Medical College, Haikou, 571101, China

    X. Z. Gao

  3. Analysis and Testing Center, Chinese Academy of Tropical Agricultural Science, Haikou, 571101, China

    P. Zhou

Authors
  1. P. Yan
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  2. X. Z. Gao
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  3. W. T. Shen
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  4. P. Zhou
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Corresponding author

Correspondence to P. Zhou.

Additional information

P. Yan and X. Z. Gao contributed equally to this work.

An erratum to this article can be found at http://dx.doi.org/10.1007/s11033-012-1878-3

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Fig. 1

Phylogenetic analysis of PDS and ZDS proteins. The neighbor-joining method was used to construct the tree. (JPG 36 kb)

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Yan, P., Gao, X.Z., Shen, W.T. et al. Cloning and expression analysis of phytoene desaturase and ζ-carotene desaturase genes in Carica papaya . Mol Biol Rep 38, 785–791 (2011). https://doi.org/10.1007/s11033-010-0167-2

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  • DOI: https://doi.org/10.1007/s11033-010-0167-2

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