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Disturbed tooth germ development in the absence of MINT in the cultured mouse mandibular explants

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Abstract

The Msx2-interacting nuclear target protein (MINT) is a nuclear matrix protein that regulates the development of many tissues. However, little is known regarding the role of MINT in tooth development. In this study, we prepared polyclonal antibodies against MINT, and found that that MINT was expressed in different cells at each stage of tooth germ development by immunohistochemistry. The role of MINT in tooth development was further illustrated by the misshapen and severely hypoplastic tooth organ in the cultured mandibular explants of MINT deficient mice. From the initiation to cap stage, the differences between mutants and wild-type molars were more and more distinguished histologically. In the MINT-deficient mandibular explants, the tooth germ was reduced in the overall size and lacked enamel knot, with abnormal dental lamina and collapsed stellate reticulum. Furthermore, the BrdU incorporation experiment showed that the proliferation activity was significantly reduced in MINT-deficient dental epithelium. Our results suggest that MINT plays an important role in tooth development, in particular, epithelial morphogenesis.

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Acknowledgements

This work was supported by grants from the National Natural Science Foundation of China (30370598, 30400079, 30600342).

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Authors and Affiliations

  1. Department of General and Emergency Dentistry, College of Stomatology, Xian, China

    Ming-Hui Zhu, Wen-Bo Dong, Guang-Ying Dong, Yong-Jin Chen & Bu-Ling Wu

  2. Department of Medical Genetics and Developmental Biology, Fourth Military Medical University, Chang-Le Xi Street #17, Xian, 710032, China

    Ming-Hui Zhu, Ping Zhang & Hua Han

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  1. Ming-Hui Zhu
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  2. Wen-Bo Dong
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  7. Hua Han
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Correspondence to Hua Han.

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Ming-Hui Zhu, Wen-Bo Dong, Guang-Ying Dong and Ping Zhang contributed equally to this work.

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Zhu, MH., Dong, WB., Dong, GY. et al. Disturbed tooth germ development in the absence of MINT in the cultured mouse mandibular explants. Mol Biol Rep 38, 777–784 (2011). https://doi.org/10.1007/s11033-010-0166-3

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  • DOI: https://doi.org/10.1007/s11033-010-0166-3

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