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Molecular characterization, polymorphism of bovine ZBTB38 gene and association with body measurement traits in native Chinese cattle breeds

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Abstract

Zinc finger and BTB domain containing 38 (ZBTB38), binding to and repressing methylated DNA, is an important candidate gene for selection of body measurement traits through marker-assisted selection (MAS). The expression of ZBTB38 is regulated in human and animal height as well as other stature indexes. Genomic structural analysis shows that bovine ZBTB38 shares much similarity with human ZBTB38. We discovered and evaluated the potential association of the single nucleotide polymorphism (SNP) of the bovine ZBTB38 gene with body measurement traits in 722 individuals. The latest findings demonstrate that the A841G SNP in exon 1 is significantly associated with Body Length (BL), Hip Height (HH) and Heart Girth (HG). Furthermore, the analysis of A841G SNP marker shows that there are significant effects on the BL (P = 0.0389) in 722 individuals, significant effects on the HH (P = 0.0173) and HG (P = 0.0147) in Qinchuan improvement steers (QI) population, as well as significant effects on the WH (P = 0.0094) in Xuelong (XL) population. These results clearly suggest that the ZBTB38 gene is among of target genes for body measurement traits in bovine reproduction and breeding, and thus provide data for establishment of an animal model using cattle to study big animal body type.

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References

  1. Sasai N, Matsuda E, Sarashina E, Ishida Y, Kawaichi M (2005) Identification of a novel BTB-zinc finger transcriptional repressor, CIBZ, that interacts with CtBP corepressor. Genes Cells 10:871–885

    Article  CAS  PubMed  Google Scholar 

  2. Kiefer H, Hermitte FC, Ravassard P, Bayard E, Brunet I, Mallet J (2005) ZENON, a novel POZ Kruppel-like DNA binding protein associated with differentiation and/or survival of late postmitotic neurons. Mol Cell Biol 25:1713–1729

    Article  CAS  PubMed  Google Scholar 

  3. Filion GJ, Zhenilo S, Salozhin S, Yamada D, Prokhortchouk E, Defossez PA (2006) A family of human zinc finger proteins that bind methylated DNA and repress transcription. Mol Cell Biol 26:169–181

    Article  CAS  PubMed  Google Scholar 

  4. Binder G, Seidel AK, Martin DD, Schweizer R, Schwarze CP, Wollmann HA, Eggermann T, Ranke MB (2008) The endocrine phenotype in Silver-Russell syndrome is defined by the underlying epigenetic alteration. J Clin Endocrinol Metab 93:1042–1047

    Article  Google Scholar 

  5. Oikawa Y, Matsuda E, Nishii T, Ishida Y, Kawaichi M (2008) Down-regulation of CIBZ, a novel substrate of caspase-3, induces apoptosis. J Biol Chem 283:14242–14247

    Article  CAS  PubMed  Google Scholar 

  6. Gudbjartsson DF, Walters GB, Thorleifsson G, Stefansson H, Halldorsson BV, Zusmanovich P, Sulem P, Thorlacius S, Gylfason A, Steinberg S, Helgadottir A, Ingason A, Steinthorsdottir V, Olafsdottir EJ, Olafsdottir GH, Jonsson T, Borch-Johnsen K, Hansen T, Andersen G, Jorgensen T, Pedersen O, Aben KK, Witjes JA, Swinkels DW, den Heijer M, Franke B, Verbeek AL, Becker DM, Yanek LR, Becker LC, Tryggvadottir L, Rafnar T, Gulcher J, Kiemeney LA, Kong A, Thorsteinsdottir U, Stefansson K (2008) Many sequence variants affecting diversity of adult human height. Nat Genet 40:609–615

    Article  CAS  PubMed  Google Scholar 

  7. Zhou G, Dudgeon C, Li M, Cao Y, Zhang L, Jin H (2010) Molecular cloning of the HGD gene and association of SNPs with meat quality traits in Chinese red cattle. Mol Biol Rep 37:603–611

    Article  CAS  PubMed  Google Scholar 

  8. Liu H, Tian W, Zan L, Wang H, Cui H (2010) Mutations of MC4R gene and its association with economic traits in Qinchuan cattle. Mol Biol Rep 37:535–540

    Article  CAS  PubMed  Google Scholar 

  9. Huang J, Wang H, Wang C, Li J, Li Q, Hou M, Zhong J (2010) Single nucleotide polymorphisms, haplotypes and combined genotypes of lactoferrin gene and their associations with mastitis in Chinese Holstein cattle. Mol Biol Rep 37:477–483

    Article  CAS  PubMed  Google Scholar 

  10. Eberlein A, Kalbe C, Goldammer T, Brunner RM, Kuehn C, Weikard R (2010) Annotation of novel transcripts putatively relevant for bovine fat metabolism. Mol Biol Rep, (in press)

  11. Xin Y, Zan L, Liu Y, Liu H, Tian W, Fan Y, Huang L (2010) Population genetic analysis of 6 Y-STR loci in Chinese northwestern Qinchuan yellow cattle breed. Mol Biol Rep, (in press)

  12. Hu J, Zhou H, Smyth A, Luo Y, Hickford JG (2010) Polymorphism of the bovine ADRB3 gene. Mol Biol Rep, (in press)

  13. Gilbert RP, Bailey DRC, Shannon NH (1993) Linear body measurements of cattle before and after 20 years of selection for post weaning gain when fed two different diets. J Anim Sci 71:1712–1720

    CAS  PubMed  Google Scholar 

  14. Mullenbach R, Lagoda PJ, Welter C (1989) An efficient salt-chloroform extraction of DNA from blood and tissues. Trends Genet 5:391

    CAS  PubMed  Google Scholar 

  15. Bundock PC, Cross MJ, Shapter FM, Henry RJ (2006) Robust allele-specific polymerase chain reaction markers developed for single nucleotide polymorphisms in expressed barley sequences. Theor Appl Genet 112:358–365

    Article  CAS  PubMed  Google Scholar 

  16. Imyanitov EN, Buslov KG, Suspitsin EN, Kuligina ES, Belogubova EV, Grigoriev MY, Togo AV, Hanson KP (2002) Improved reliability of allele-specific PCR. Biotechniques 33:484–490

    CAS  PubMed  Google Scholar 

  17. Nasis O, Thompson S, Hong T, Sherwood M, Radcliffe S, Jackson L, Otevrel T (2004) Improvement in sensitivity of allele-specific PCR facilitates reliable noninvasive prenatal detection of cystic fibrosis. Clinical Chem 50:694–701

    Article  CAS  Google Scholar 

  18. Nei M, Roychoudhury AK (1974) Sampling variance of heterozygosity and genetic distance. Genetics 76:379–390

    CAS  PubMed  Google Scholar 

  19. Nei M, Li WH (1979) Mathematic model for studying genetic variation in terms of restriction endonucleaes. Pnas 76:5269–5273

    Article  CAS  PubMed  Google Scholar 

  20. Liu YF, Zan LS, Li K, Zhao SP, Xin YP, Lin Q, Tian WQ, Wang ZW (2010) A novel polymorphism of GDF5 gene and its association with body measurement traits in Bos taurus and Bos indicus breeds. Mol Biol Rep 37:429–434

    Article  CAS  PubMed  Google Scholar 

  21. Mateescu RG, Zhang Z, Tsai K, Phavaphutanon J, Burton-wurster NI, Quaas R, Murphy K, Acland GM, Todhunter RJ (2005) Analysis of allele fidelity, polymorphic information content, and density of microsatellites in a genome-wide screening for hip dysplasia in a crossbreed pedigree. J Here 96:847–853

    Article  CAS  Google Scholar 

  22. Ren G, Chen H, Zhang LZ, Lan XY, Wei TB, Li MJ, Jing YJ, Lei CZ, Wang JQ (2010) A coding SNP of LHX4 gene is associated with body weight and body length in bovine. Mol Biol Rep 37:417–422

    Article  CAS  PubMed  Google Scholar 

  23. Wang J, Li ZJ, Lan XY, Hua LS, Huai YT, Huang YZ, Ma L, Zhao M, Jing YJ, Chen H, Wang JQ (2010) Two novel SNPs in the coding region of the bovine PRDM16 gene and its associations with growth traits. Mol Biol Rep 37:571–577

    Article  CAS  PubMed  Google Scholar 

  24. Li F, Chen H, Lei CZ, Ren G, Wang J, Li ZJ, Wang JQ (2010) Novel SNPs of the bovine GAD1/gad67 gene and their association with growth traits in three native Chinese cattle breeds. Mol Biol Rep 37:501–505

    Article  CAS  PubMed  Google Scholar 

  25. Li F, Chen H, Lei CZ, Ren G, Wang J, Li ZJ, Wang JQ (2010) Novel SNPs of the bovine NUCB2 gene and their association with growth traits in three native Chinese cattle breeds. Mol Biol Rep 37:541–546

    Article  CAS  PubMed  Google Scholar 

  26. Jin QJ, Sun JJ, Fang XT, Zhang CL, Yang L, Chen DX, Shi XY, Du Y, Lan XY, Chen H (2010) Molecular characterization and polymorphisms of the caprine Somatostatin (SST) and SST Receptor 1 (SSTR1) genes that are linked with growth traits. Mol. Biol. Rep., inpress

  27. Sun J, Jin Q, Zhang C, Fang X, Gu C, Lei C, Wang J, Chen H (2010) Polymorphisms in the bovine ghrelin precursor (GHRL) and Syndecan-1 (SDC1) genes that are associated with growth traits in cattle. Mol. Biol. Rep., inpress

  28. Lettre GA, Jackson AU, Gieger C, Schumacher FR, Berndt SI, Sanna S, Eyheramendy S, Voight BF, Butler JL, Guiducci C, Illig T, Hackett R, Heid IM, Jacobs KB, Lyssenko V, Uda M, Boehnke M, Chanock SJ, Groop LC, Hu FB, Isomaa B, Kraft P, Peltonen L, Salomaa V, Schlessinger D, Hunter DJ, Hayes RB, Abecasis GR, Wichmann HE, Mohlke KL, Hirschhorn JN (2008) Identification of ten loci associated with height highlights new biological pathways in human growth. Nat Genet 40:584–591

    Article  CAS  PubMed  Google Scholar 

  29. Weedon MN, Lango H, Lindgren CM, Wallace C, Evans DM, Mangino M, Freathy RM, Perry JRB, Stevens S, Hall AS, Samani NJ, Shields B, Prokopenko I, Farrall M, Dominiczak A, Johnson T, Bergmann S, Beckmann JS, Vollenweider P, Waterworth DM, Mooser V, Palmer CNA, Morris AD, Ouwehand WH, Caulfield M, Munroe PB, Hattersley AT, McCarthy MI, Frayling TM (2008) Genome-wide association analysis identifies 20 loci that influence adult height. Nat Genet 40:575–583

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

We thank Dr Baoli Hu of Dana-Farber Cancer Institute, Boston MA, USA, for critically reading and discussing this manuscript. This work was supported by the China National “863” Program (2006AA10Z1A1), the National Eleventh “Five-Year” Science and Technology Support Project (2006BAD01A10-3) and National Key Scientific Program (2006CB102105, 2009CB941604). Moreover, the bovine populations were supported by Excellent research team of livestock gene resource and breed improvement of Chinese academy of agricultural sciences, Qinchuan beef cattle breeding center of Shaanxi province, Nanyang, Jiaxian and Xianan cattle breeding center of Henan province, Luxi cattle breeding center of Shandong province, and Snow dragon beef cattle breeding center of Liaoning province (PR China).

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Authors and Affiliations

  1. College of Animal Science and Technology, Northwest A & F University, No. 22 Xinong Road, 712100, Yangling, Shaanxi, People’s Republic of China

    Yongfeng Liu, Linsen Zan, Shuanping Zhao, Yaping Xin & Linqiang Li

  2. Key Laboratory for Farm Animal Genetic Resources and Utilization of Ministry of Agriculture of China, Institute of Animal Science, Chinese Academy of Agricultural Sciences, 100193, Beijing, People’s Republic of China

    Yongfeng Liu, Wentao Cui, Zhonglin Tang & Kui Li

  3. National Beef cattle improvement Centre of China, 712100, Yangling, Shaanxi, People’s Republic of China

    Linsen Zan

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  1. Yongfeng Liu
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  2. Linsen Zan
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  4. Yaping Xin
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Correspondence to Linsen Zan or Kui Li.

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Liu, Y., Zan, L., Zhao, S. et al. Molecular characterization, polymorphism of bovine ZBTB38 gene and association with body measurement traits in native Chinese cattle breeds. Mol Biol Rep 37, 4041–4049 (2010). https://doi.org/10.1007/s11033-010-0063-9

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  • DOI: https://doi.org/10.1007/s11033-010-0063-9

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