Abstract
Two cotton vacuolar-invertase genes were identified and sequenced. Both genes had seven exons, including an unusually small second exon typical of acid invertases. These genes encode peptides with many features shared by acid invertases from other species including, leader sequences that probably target the peptide to the vacuole, active site motifs and substrate binding motifs. Expression analyses indicated that one of the genes was expressed in roots during the starch filling stage of development. However, expression of the same gene fluctuated during the starch utilization stage of development. Therefore this gene was unlikely to play a role in determining sink strength of this tissue. Both genes were expressed in elongating fibers where they were likely to play a role in cell expansion. The invertase gene uniquely expressed in fiber had a simple sequence repeat (SSR) in the third intron that was polymorphic among various cotton species. An EST was identified with an expansion of the SSR that included the third intron indicating this SSR is associated with a splice variant. The polymorphic SSR may be useful in investigating the function of this gene in fiber development.
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Acknowledgements
The author thanks Pameka Johnson for excellent technical assistance and Drs. Ricki Turley and Candace Haigler for critical review of this manuscript. This work was supported by USDA/ARS CRIS number 6402-21000-029-00. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA.
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Taliercio, E., Scheffler, J. & Scheffler, B. Characterization of two cotton (Gossypium hirsutum L) invertase genes. Mol Biol Rep 37, 3915–3920 (2010). https://doi.org/10.1007/s11033-010-0048-8
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DOI: https://doi.org/10.1007/s11033-010-0048-8