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Identification and characterization of adipose triglyceride lipase (ATGL) gene in birds

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Abstract

Adipose triglyceride lipase (ATGL) is a triglyceride hydrolysis lipase and is generally related to lipid metabolism in animals. The ATGL gene was well studied in mammals, however very less was known in birds that differed significantly with mammals for lipid metabolism. In this study, cloning, mRNA real time and association analysis was performed to characterize the ATGL gene in birds. Results showed that the obtained ATGL gene cDNA of parrot, quail, duck were 1,651 bp (NCBI accession number: GQ221784), 1,557 bp (NCBI accession number: GQ221783) and 1,440 bp each, encoded 481-, 482- and 279-amino acid (AA) peptide, respectively. The parrot ATGL (pATGL) gene was found to predominantly express in breast muscle and leg muscle, and very higher ATGL mRNA level was also found in heart, abdominal fat and subcutaneous fat. The quail ATGL (qATGL) gene was also predominantly expressed in breast muscle and leg muscle, and then to a much lesser degree in heart. The duck ATGL (dATGL) gene was found to predominantly express in subcutaneous fat and abdominal fat, quite higher ATGL mRNA was also found in heart, spleen, breast muscle and leg muscle. Blast analyses indicated the high homology of ATGL and its patatin region, and moreover, and the active serine hydrolase motif (“GASAG” for “GXSXG”) and the glycine rich motif (“GCGFLG” for “GXGXXG”) were completely conservative among 14 species. Association analyses showed that c.950+24C>A, c.950+45C>G, c.950+73G>A, c.950+83C>T and c.950+128delA of chicken ATGL gene (cATGL) were all significantly or highly significantly with cingulated fat width (CFW) (P < 0.05 or P < 0.01), and c.777−26C>A, c.950+45C>G, c.950+73G>A and c.950+118C>T were all significantly or highly significantly with pH value of breast muscle (BMPH) (P < 0.05).

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Acknowledgments

This work was funded by projects under the Major State Basic Research Development Program, China, project no. 2006CB102100, and the National Natural Science Foundation of China (project number: 30600429).

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Authors and Affiliations

  1. Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, 510642, Guangzhou, Guangdong, China

    Qinghua Nie, Yongsheng Hu, Liang Xie, Chengguang Zhang, Xu Shen & Xiquan Zhang

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  1. Qinghua Nie
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  2. Yongsheng Hu
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  3. Liang Xie
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  4. Chengguang Zhang
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  5. Xu Shen
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Correspondence to Xiquan Zhang.

Additional information

Yongsheng Hu, Liang Xie, Chengguang Zhang contributed equally to this work.

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Nie, Q., Hu, Y., Xie, L. et al. Identification and characterization of adipose triglyceride lipase (ATGL) gene in birds. Mol Biol Rep 37, 3487–3493 (2010). https://doi.org/10.1007/s11033-009-9941-4

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  • DOI: https://doi.org/10.1007/s11033-009-9941-4

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