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Enhanced salt tolerance of transgenic poplar plants expressing a manganese superoxide dismutase from Tamarix androssowii

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Abstract

Superoxide dismutases (SODs) play important role in stress tolerance of plants. In this study, an MnSOD gene (TaMnSOD) from Tamarix androssowii, under the control of the CaMV35S promoter, was introduced into poplar (Populus davidiana × P. bolleana). The physiological parameters, including SOD activity, malondialdehyde (MDA) content, relative electrical conductivity (REC) and relative weight gain, of transgenic lines and wild type (WT) plants, were measured and compared. The results showed that SOD activity was enhanced in transgenic plants, and the MDA content and REC were significantly decreased compared to WT plants when exposed to NaCl stress. In addition, the relative weight gains of the transgenic plants were 8- to 23-fold of those observed for WT plants after NaCl stress for 30 days. The data showed that the SOD activities that increased in transgenic lines are 1.3–4-folds of that increased in the WT plant when exposed to NaCl stress. Our analysis showed that increases in SOD activities as low as 0.15-fold can also significantly enhance salt tolerance in transgenic plants, suggesting an important role of increased SOD activity in plant salt tolerance.

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Acknowledgments

This work was supported by the Key Project of Science–Technology Research granted by the Ministry of Education of China (No. 107037), Natural Science Foundation of Heilongjiang province (No. QC07C56), and the Key Research Projects of Heilongjiang Province (No. WB07N02).

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Authors and Affiliations

  1. Key Laboratory of Forest Tree Genetic Improvement and Biotechnology (Northeast Forestry University), Ministry of Education, 26 Hexing Road, 150040, Harbin, China

    Yu Cheng Wang, Guan Zheng Qu, Ying Jie Wu, Chao Wang, Gui Feng Liu & Chuan Ping Yang

  2. Heilongjiang Academy of Forestry, 134 Haping Road, 150040, Harbin, China

    Hong Yan Li

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  1. Yu Cheng Wang
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  2. Guan Zheng Qu
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  3. Hong Yan Li
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  4. Ying Jie Wu
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  5. Chao Wang
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  6. Gui Feng Liu
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  7. Chuan Ping Yang
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Correspondence to Chuan Ping Yang.

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Wang, Y.C., Qu, G.Z., Li, H.Y. et al. Enhanced salt tolerance of transgenic poplar plants expressing a manganese superoxide dismutase from Tamarix androssowii . Mol Biol Rep 37, 1119–1124 (2010). https://doi.org/10.1007/s11033-009-9884-9

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  • DOI: https://doi.org/10.1007/s11033-009-9884-9

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