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Down-regulation of Pkd2 by siRNAs suppresses cell–cell adhesion in the mouse melanoma cells

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Abstract

The Pkd2 gene encodes an integral protein (~130 kDa), named polycystin-2 (PC-2). PC-2 is mainly involved in autosomal dominant polycystic kidney disease. Recently, polycystin-1/polycystin-2 complex has been shown to act as an adhesion complex mediating or regulating cell–cell or cell–matrix adhesion, suggesting that PC-2 may play a role in cell–cell/cell–matrix interactions. Here, we knocked down the expression of Pkd2 gene with small interfering RNAs (siRNAs) in the mouse melanoma cells (B16 cells), indicating that the cells transfected with the targeted siRNAs significantly suppressed cell–cell adhesion, but not cell–matrix adhesion, compared to the cells transfected with non-targeted control (NC) siRNA. This study provides the first directly functional evidence that PC-2 mediates cell–cell adhesion. Furthermore, we demonstrated that PC-2 modulated cell–cell adhesion may be, at least partially, associated with E-cadherin. Collectively, these findings for the first time showed that PC-2 may mediate cell–cell adhesion, at least partially, through E-cadherin.

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Acknowledgments

We wish to acknowledge the grant from National Key Basic Research Program of China (2005CB522506) for Prof. Qin Zhou M.D.&Ph.D, who was a recipient of the Initial Foundation of M.O.E. (Ministry of Education) for Returned Overseas Students (20071108-18-18). Ge Cao is a recipient of the National Natural Science Foundation of China (30801134). The work was also supported by a grant from National Key Basic Research Program of China to academician Yuquan Wei (2004CB518800).

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Authors and Affiliations

  1. Core Facility of Gene Engineered Mice, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Keyuan the Fourth Road, The District of Hi&Tech, 610041, Chengdu, People’s Republic of China

    Guo-Hui Bian, Qing-Wei Li, Huan Sun, Yan Xiao, Jian-Zhong Ai, Qui-Tan Yang, Jing-Jing Duan, Yi-Dong Wang, Zheng Zhang, Rui-Zhi Tan, Yu-Hang Liu, Yang Yang, Yu-Quan Wei & Qin Zhou

  2. Department of Thoracic & Cardiovascular Surgery, West China Hospital, Sichuan University, 610041, Sichuan, People’s Republic of China

    Ge Cao

  3. Department of Dermatology, West China Hospital, Sichuan University, 610041, Sichuan, People’s Republic of China

    Xiao-Yan Lv

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  1. Guo-Hui Bian
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Correspondence to Qin Zhou.

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Guo-Hui Bian and Ge Cao contributed equally to this work.

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Bian, GH., Cao, G., Lv, XY. et al. Down-regulation of Pkd2 by siRNAs suppresses cell–cell adhesion in the mouse melanoma cells. Mol Biol Rep 37, 2387–2395 (2010). https://doi.org/10.1007/s11033-009-9746-5

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  • DOI: https://doi.org/10.1007/s11033-009-9746-5

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