Abstract
Our previous reports indicated that cyclic AMP response element-binding (CREB) protein was involved in the regulation of Bcl-2 expression in apoptotic HeLa cells induced by trichosanthin (TCS). Here we presented that blockade the binding site of CREB to Bcl-2 by a CRE decoy oligonucleotide abrogated the TCS-decreased Bcl-2 expression. Furthermore, overexpression of phosphorylated CREB (p-CREB) in cells transfected with p-CREB/GFP fusion construct resulted in an increase of Bcl-2 protein content, however, this increase was attenuated by TCS treatment. Therefore, this data supports the hypothesis that CREB is a possible upstream regulator of Bcl-2 in apoptotic HeLa cells induced by TCS. The study provides new insights into understanding the mechanism of TCS in the treatment of cervical cancer.
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Acknowledgments
This work was supported by the grants from the National Natural Science Foundation of China (30800577), Qianjiang Talent Project of Zhejiang Province of China (NO. 2009R10057) and K. C. Wong Magna Fund in Ningbo University.
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Wang, P., Xu, J. & Zhang, C. CREB, a possible upstream regulator of Bcl-2 in trichosanthin-induced HeLa cell apoptosis. Mol Biol Rep 37, 1891–1896 (2010). https://doi.org/10.1007/s11033-009-9629-9
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DOI: https://doi.org/10.1007/s11033-009-9629-9