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The involvement of HSP22 from bay scallop Argopecten irradians in response to heavy metal stress

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Abstract

Heat shock protein 22 (HSP22) is an important member of small heat shock protein (sHSP) subfamily which plays a key role in the process of protecting cells, facilitating the folding of nascent peptides, and responding to stress. In the present study, the cDNA of HSP22 was cloned from Argopecten irradians (designated as AiHSP22) by rapid amplification cDNA end (RACE) based on the expressed sequence tags (ESTs). The full-length cDNA of AiHSP22 was of 1,112 bp, with an open reading frame of 588 bp encoding a polypeptide of 195 amino acids. The deduced amino acid sequence of AiHSP22 showed high similarity to previously identified HSP22s. The expression patterns of AiHSP22 mRNA in different tissues and in haemocytes of scallops exposed to Cd2+, Pb2+ or Cu2+ were investigated by real-time quantitative RT-PCR. The mRNA of AiHSP22 was constitutively expressed in all examined tissues, including haemocyte, muscle, kidney, gonad, gill and heart. The expression level in heart and muscle was higher than that in other tissues. The mRNA level of AiHSP22 in haemocytes was up-regulated after a 10 days exposure of scallops to Cu2+, Pb2+ and Cd2+. However, the expression of AiHSP22 did not increase linearly along with the rise of heavy metal concentration. Different concentrations of the same metal resulted in different effects on AiHSP22 expression. The sensitive response of AiHSP22 to Cu2+, Pb2+ and Cd2+ stress indicated that it could be developed as an indicator of exposure to heavy metals for the pollution monitoring programs in aquatic environment.

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Acknowledgments

The authors would like to thank all laboratory members for technical advice and helpful discussions. This research was supported by 863 High Technology Project (No. 2006AA10A402) from the Chinese Ministry of Science and Technology, and a commonweal project (No. nyhyzx07-047) from the Chinese Ministry of Agriculture, and grants (No. 30730070) from NSFC to Dr. Linsheng Song.

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Authors and Affiliations

  1. College of Marine Life, Ocean University of China, 266003, Qingdao, China

    Lei Zhang, Chaohua Dong & Guanpin Yang

  2. The Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Rd., 266071, Qingdao, China

    Lei Zhang, Lingling Wang, Linsheng Song, Jianmin Zhao, Limei Qiu, Chaohua Dong, Fengmei Li & Huan Zhang

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  1. Lei Zhang
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  2. Lingling Wang
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Correspondence to Lingling Wang or Linsheng Song.

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Zhang, L., Wang, L., Song, L. et al. The involvement of HSP22 from bay scallop Argopecten irradians in response to heavy metal stress. Mol Biol Rep 37, 1763–1771 (2010). https://doi.org/10.1007/s11033-009-9603-6

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  • DOI: https://doi.org/10.1007/s11033-009-9603-6

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